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Understanding Cancer Series

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  • Posted: 10/20/2009

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Slide 4


Genome-Wide Profiling: Many Ways

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Genome-wide profiling refers to the collection of large-scale information about the sequence and expression of the genes in normal or tumor cells. Techniques include:

  • Comparative Genomic Hybridization and Spectral Karyotyping
    looking at global changes in chromosome structure when cancer cells are compared to normal ones from the same patient
  • Polymorphism Analysis
    looking at genetic variations called polymorphisms in individuals and in populations (These specific altered regions or sequences are spread across the entire genome.)
  • Gene Expression Profiling
    capturing total gene activities, both increases and decreases, across a genome as patterns of gene expression

Most of these techniques use high-throughput technologies, which are automated systems that can perform rapid analysis of large numbers of samples. Data generated through genome-wide profiling are sometimes called genomic signatures or genomic profiles.

Graphic shows four clinicians all holding different technology used for genome-wide profiling of cancer tissue. First holds a set of chromosomes each with a different color attached. This lets the researchers see two colors where genetic material has been transferred between chromosomes.