The NCI RAS Initiative has a "hub-and-spokes" structure, in that its scientists work both independently and in collaboration with academic and commercial entities. The list of RAS Initiative publications below reflects the balance between the public responsibilities of the National Cancer Institute and the requirements of our external partners.
Authors who names are marked with an asterisk (*) are part of the RAS Initiative.
- RASopathy mutations provide functional insight into the BRAF cysteine-rich domain and reveal the importance of autoinhibition in BRAF regulation
Spencer-Smith R, Terrell EM, Insinna C, Agamasu C*, Wagner ME*, Ritt DA, Stauffer J, Stephen AG*, Morrison DK
Mol Cell. 2022;82(22):4262-76 e5
PubMed PMID: 36347258
- Classical RAS proteins are not essential for paradoxical ERK activation induced by RAF inhibitors
Lai LP*, Fer N*, Burgan W*, Wall V*, Xu B*, Soppet D*, Esposito D*, Nissley DV*, McCormick F
Proc Natl Acad Sci U S A 2022
PubMed PMID: 35091470
- Insights into the cross talk between effector and allosteric lobes of KRAS from methyl conformational dynamics
Chao FA*, Dharmaiah S*, Taylor T*, Messing S*, Gillette W*, Esposito D*, Nissley DV*, McCormick F, Byrd RA, Simanshu DK*, Cornilescu G*
J Am Chem Soc 2022
PubMed PMID: 35213144
- Machine learning-driven multiscale modeling reveals lipid-dependent dynamics of RAS signaling proteins
Ingólfsson HI, Neale C, Carpenter TS, Shrestha R*, López CA, Tran TH*, Oppelstrup T, Bhatia H, Stanton LG, Zhang X, Sundram S, Di Natale F, Agarwal A, Dharuman G, Kokkila Schumacher SIL, Turbyville T*, Gulten G*, Van QN*, Goswami D*, Jean-Francois F*, Agamasu C*, Chen D*, Hettige JJ, Travers T, Sarkar S, Surh MP, Yang Y, Moody A, Liu S, Van Essen BC, Voter AF, Ramanathan A, Hengartner NW, Simanshu DK*, Stephen AG*, Bremer PT, Gnanakaran S, Glosli JN, Lightstone FC, McCormick F, Nissley DV*, Streitz FH
Proc Natl Acad Sci USA 2022
PubMed PMID: 34983849
- The tumor suppressor activity of DLC1 requires the interaction of its START domain with phosphatidylserine, PLCD1, and caveolin-1
Sanchez-Solana B, Wang D, Qian X, Velayoudame P, Simanshu DK*, Acharya JK, Lowy DR
Mol Cancer 2021
PubMed PMID: 34727930
- NMR 1H,13C, 15N backbone resonance assignments of the T35S and oncogenic T35S/Q61L mutants of human KRAS4b in the active, GppNHp-bound conformation
Sharma AK*, Dyba M*, Tonelli M, Smith B*, Gillette WK*, Esposito D*, Nissley DV*, McCormick F, Maciag AE*
Biomol NMR Assign 2021
PubMed PMID: 34686998
- Recapitulation of cell-like KRAS4b membrane dynamics on complex biomimetic membranes
Shrestha R*, Chen D*, Frank P*, Nissley DV*, Turbyville TJ*
iScience 2021
PubMed PMID: 35106460
- Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinant leucine-rich repeat proteins and protein complexes
Snead K*, Wall V*, Ambrose H*, Esposito D*, Drew M*
Protein Expr Purif 2022
PubMed PMID: 35131438
- Virtual screening for small-molecule pathway regulators by image-profile matching
Rohban MH, Fuller AM, Tan C, Goldstein JT, Syangtan D, Gutnick A, DeVine A, Nijsure MP, Rigby M, Sacher JR, Corsello SM, Peppler GB, Bogaczynska M, Boghossian A, Ciotti GE, Hands AT, Mekareeya A, Doan M, Gale JP, Derynck R, Turbyville T*, Boerckel JD, Singh S, Kiessling LL, Schwarz TL, Varelas X, Wagner FF, Kafri R, Eisinger-Mathason TSK, Carpenter AE
Cell Syst. 2022;13(9):724-36 e9
PubMed PMID: 36057257
- RAS at 40: Update from the RAS initiative
Nissley DV*, McCormick F
Cancer Discov 2022
PubMed PMID: 35046096
- Exploring CRD mobility during RAS/RAF engagement at the membrane
Nguyen K, López CA, Neale C, Van QN*, Carpenter TS, Di Natale F, Travers T, Tran TH*, Chan AH*, Bhatia H, Frank PH, Tonelli M, Zhang X, Gulten G*, Reddy T, Burns V, Oppelstrup T, Hengartner N, Simanshu DK*, Bremer PT, Chen D*, Glosli JN, Shrestha R*, Turbyville T*, Streitz FH, Nissley DV*, Ingólfsson HI, Stephen AG*, Lightstone FC, Gnanakaran S.
Biophys J. 2022;121(19):3630-50
PubMed PMID: 35778842
- Asynchronous Reciprocal Coupling of Martini 2.2 Coarse-Grained and CHARMM36 All-Atom Simulations in an Automated Multiscale Framework
López CA, Zhang X, Aydin F, Shrestha R*, Van QN*, Stanley CB, Carpenter TS, Nguyen K, Patel LA, Chen D*, Burns V, Hengartner NW, Reddy TJE, Bhatia H, Di Natale F, Tran TH*, Chan AH*, Simanshu DK*, Nissley DV*, Streitz FH, Stephen AG*, Turbyville TJ*, Lightstone FC, Gnanakaran S, Ingólfsson HI, Neale C
J Chem Theory Comput. 2022;18(8):5025-45
PubMed PMID: 35866871
- A structure is worth a thousand words: New insights for RAS and RAF regulation
Simanshu DK*, Morrison DK
Cancer Discov 2022
PubMed PMID: 35046094
- Structure of the SHOC2-MRAS-PP1C complex provides insights into RAF activation and Noonan syndrome
Bonsor DA*, Alexander P*, Snead K*, Hartig N, Drew M*, Messing S*, Finci LI*, Nissley DV*, McCormick F, Esposito D*, Rodriguez-Viciana P, Stephen AG*, Simanshu DK*
Nat Struct Mol Biol. 2022;29(10):966-77
PubMed PMID: 36175670
- Mapping the KRAS Proteoform Landscape in Colorectal Cancer Identifies Truncated KRAS4B that Decreases MAPK Signaling
Adams LM, DeHart CJ*, Drown BS, Anderson LC, Bocik W, Boja ES, Hiltke TM, Hendrickson CL, Rodriguez H, Caldwell M, Vafabakhsh R, Kelleher NL
J Biol Chem. 2022:102768
PubMed PMID: 36470426
- RAS interaction with Sin1 is dispensable for mTORC2assembly and activity
Castel P, Dharmaiah S*, Sale MJ, Messing S*, Rizzuto G, Cuevas-Navarro A, Cheng A, Trnka MJ, Urisman A, Esposito D*, Simanshu DK*, McCormick F
Proc Natl Acad Sci U S A 2020
PubMed PMID: 34380736
- Refining the N-termini of the SARS-CoV-2 spike protein and its discrete receptor binding domain
D’Ippolito RA*, Drew MR*, Mehalko J*, Snead K*, Wall V*, Putman Z*, Esposito D*, DeHart CJ*
J Proteome Res 2021
PubMed PMID: 34379411
- Sensitivity of oncogenic KRAS-expressing cells to CDK9 inhibition
Lai LP*, Brel V, Sharma K*, Frappier J, Le-Henanf N, Vivet B, Muzet N, Schell E, Morales R, Rooney E, Basse N, Yi M*, Lacroix F, Holderfield M*, Englaro W, Marcireau C, Debussche L, Nissley DV*, McCormick F
SLAS Discov 2021
PubMed PMID: 33896272
- Production and membrane binding of N-terminally acetylated, C-terminally farnesylated and carboxymethylated KRAS4b
Messing S*, Agamasu C*, Drew M*, DeHart CJ*, Stephen AG*, Gillette WK*
Methods Mol Biol 2021
PubMed PMID: 33977473
- The metabolic landscape of RAS-driven cancers from biology to therapy
Mukhopadhyay S*, Vander Heiden MG, McCormick F*
Nat Cancer 2021
PubMed PMID: 33870211
- Unveiling the Dynamics of KRAS4b on Lipid Model Membranes
Lopez CA, Agarwal A, Van QN*, Stephen AG*, Gnanakaran S.
J Membr Biol. 2021;254(2):201-16
PubMed PMID: 33825026
- Membrane-bound KRAS approximates an entropic ensemble of configurations
Heinrich F, Van QN*, Jean-Francois F*, Stephen AG*, Losche M.
Biophys J. 2021;120(18):4055-66
PubMed PMID: 34384763
- Development of a High-throughput NanoBRET Screening Platform to Identify Modulators of the RAS/RAF Interaction
Durrant DE, Smith EA, Goncharova EI, Sharma N, Alexander PA*, Stephen AG*, Henrich CJ, Morrison DK
Mol Cancer Ther. 2021;20(9):1743-54
PubMed PMID: 34158349
- NMR 1H,13C, 15N backbone resonance assignments of the T35S and oncogenic T35S/Q61L mutants of human KRAS4b in the active, GppNHp-bound conformation
Sharma AK*, Dyba M*, Tonelli M, Smith B*, Gillette WK*, Esposito D*, Nissley DV*, McCormick F, Maciag AE*
Biomol NMR Assign 2021
PubMed PMID: 34686998
- Targeting eIF4A-dependent translation of KRAS signaling molecules
Singh K, Lin J, Lecomte N, Mohan P, Gokce A, Sanghvi VR, Jiang M, Grbovic-Huezo O, Burčul A, Stark SG, Romesser PB, Chang Q, Melchor JP, Beyer RK*, Duggan M, Fukase Y, Yang G, Ouerfelli O, Viale A, de Stanchina E, Stamford AW, Meinke PT, Rätsch G, Leach SD, Ouyang Z, Wendel HG
Cancer Res 2021
PubMed PMID: 33632898
- KRAS interaction with RAF1 RAS-binding domain and cysteine-rich domain provides insights into RAS-mediated RAF activation
Tran TH*, Chan AH*, Young LC, Bindu L*, Neale C, Messing S*, Dharmaiah S*, Taylor T*, Denson JP*, Esposito D*, Nissley DV*, Stephen AG*, McCormick F*, Simanshu DK*
Nat Commun 2021
PubMed PMID: 33608534
- A practical guide to large-scale docking
Bender BJ, Gahbauer S, Luttens A, Lyu J, Webb CM, Stein RM, Fink EA, Balius TE*, Carlsson J, Irwin JJ, Shoichet BK
Nat Protoc 2021
PubMed PMID: 34561691
- A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells
Grose C*, Putman Z*, Esposito D*
Protein Expr Purif 2021
PubMed PMID: 34087362
- RAS nanoclusters: dynamic signaling platforms amenable to therapeutic intervention
Van QN*, Prakash P, Shrestha R*, Balius TE*, Turbyville TJ*, Stephen AG*
Biomolecules 2021
PubMed PMID: 33802474
- Uncovering a membrane-distal conformation of KRAS available to recruit RAF to the plasma membrane
Van QN*, López CA, Tonelli M, Taylor T*, Niu B, Stanley CB, Bhowmik D, Tran TH*, Frank PH*, Messing S*, Alexander P*, Scott D, Ye X*, Drew M*, Chertov O*, Lösche M, Ramanathan A, Gross ML, Hengartner NW, Westler WM, Markley JL, Simanshu DK*, Nissley DV*, Gillette WK*, Esposito D*, McCormick F*, Gnanakaran S, Heinrich F, Stephen AG*
Proc Natl Acad Sci U S A 2020
PubMed PMID: 32913056
- Structural Insights into the SPRED1-Neurofibromin-KRAS Complex and Disruption of SPRED1-Neurofibromin Interaction by Oncogenic EGFR
Yan W*, Markegard E, Dharmaiah S*, Urisman A, Drew M*, Esposito D*, Scheffzek K, Nissley DV*, McCormick F*, Simanshu DK*
Cell Rep 2020
PubMed PMID: 32697994
- Anionic Lipids Impact RAS-Binding Site Accessibility and Membrane Binding Affinity of CRAF RBD-CRD
Travers T, López CA, Agamasu C*, Hettige JJ, Messing S*, García AE, Stephen AG*, Gnanakaran S
Biophys J 2020
PubMed PMID: 32649863
- ssGSEA score-based Ras dependency indexes derived from gene expression data reveal potential Ras addiction mechanisms with possible clinical implications
Yi M*, Nissley DV*, McCormick F*, Stephens RM*
Sci Rep 2020
PubMed PMID: 32581224
- RAS internal tandem duplication disrupts GTPase-activating protein (GAP) binding to activate oncogenic signaling
Nelson AC, Turbyville TJ*, Dharmaiah S*, Rigby M*, Yang R, Wang TY, Columbus J*, Stephens R*, Taylor T*, Sciacca D, Onsongo G, Sarver A, Subramanian S, Nissley DV*, Simanshu DK*, Lou E
J Biol Chem 2020
PubMed PMID: 32393580
- The frequency of Ras mutations in cancer
Prior IA, Hood FE, Hartley JL*
Cancer Res 2020
PubMed PMID: 32209560
- Homogeneous dual-parametric coupled assay for simultaneous nucleotide exchange and KRAS/RAF-RBD interaction monitoring
Kopra K, Vuorinen E, Abreu-Blanco M*, Wang Q, Eskonen V, Gillette W*, Pulliainen AT, Holderfield M*, Härmä H.
Anal Chem 2020
PubMed PMID: 32106676
- The small molecule BI-2852 induces a nonfunctional dimer of KRAS.
Tran TH*, Alexander P*, Dharmaiah S*, Agamasu C*, Nissley DV*, McCormick F*, Esposito D*, Simanshu DK*, Stephen AG*, Balius TE*
Proc Natl Acad Sci U S A 2020
PubMed PMID: 32047043
- Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein
Agamasu C*, Frank P*, Perkins S*, Waybright T*, Messing S*, Gillette W*, Stephen AG*
J Vis Exp 2020
PubMed PMID: 32009649
- Membrane interactions of the globular domain and the hypervariable region of KRAS4b define its unique diffusion behavior
Goswami D*, Chen D*, Yang Y, Gudla PR*, Columbus J*, Worthy K*, Rigby M*, Wheeler M*, Mukhopadhyay S*, Powell K*, Burgan W*, Wall V*, Esposito D*, Simanshu D*, Lightstone FC, Nissley DV*, McCormick F*, Turbyville T*
Elife 2020
PubMed PMID: 31958057
- PDE6D Inhibitors with a New Design Principle Selectively Block K-Ras Activity
Siddiqui FA, Alam C, Rosenqvist P, Ora M, Sabt A, Manoharan GB, Bindu L*, Okutachi S, Catillon M, Taylor T*, Abdelhafez OM, Lönnberg H, Stephen AG*, Papageorgiou AC, Virta P, Abankwa D
ACS Omega 2019
PubMed PMID: 31956834
- Undermining glutaminolysis bolsters chemotherapy while NRF2 promotes chemoresistance in KRAS-driven pancreatic cancers
Mukhopadhyay S*, Goswami D*, Adiseshaiah PP, Burgan W*, Yi M*, Guerin TM, Kozlov SV, Nissley DV*, McCormick F*
Cancer Res 2020
PubMed PMID: 31911550
- A Massively Parallel Infrastructure for Adaptive Multiscale Simulations: Modeling RAS Initiation Pathway for Cancer
F. Di Natale, H. Bhatia, T. S. Carpenter, C. Neale, S. Kokkila Schumacher, T. Oppelstrup, L. Stanton, X. Zhang, S. Sundram, T. R. W. Scogland, G. Dharuman, M. P. Surh, Y. Yang, C. Misale, L. Schneidenbach, C. Costa, C. Kim, B. D’Amora, S. Gnanakaran, D. V. Nissley*, F. Streitz, F. C. Lightstone, P.-T. Bremer, J. N. Glosli, H. I. Ingolfsson
SC '19: Proceedings of the International Conference for High Performance Computing, Networking, Storage and Analysis, Article No.: 57, 1–16.
Winner of SC Best Paper Award
DOI: 10.1145/3295500.3356197
- Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer
Sherekar M*, Han SW, Ghirlando R, Messing S*, Drew M*, Rabara D*, Waybright T*, Juneja P, O'Neill H, Stanley CB, Bhowmik D, Ramanathan A, Subramaniam S, Nissley DV*, Gillette W*, McCormick F*, Esposito D*
J Biol Chem 2019
PubMed PMID: 31836666
- Atypical KRASG12R Mutant Is Impaired in PI3K Signaling and Macropinocytosis in Pancreatic Cancer.
Hobbs GA, Baker NM, Miermont AM, Thurman RD, Pierobon M, Tran TH*, Anderson AO, Waters AM, Diehl JN, Papke B, Hodge RG, Klomp JE, Goodwin CM, DeLiberty JM, Wang J, Ng RWS, Gautam P, Bryant KL, Esposito D*, Campbell SL, Petricoin EF, Simanshu DK*, Aguirre AJ, Wolpin BM, Wennerberg K, Rudloff U, Cox AD, Der CJ.
Cancer Discov 2019
PubMed PMID: 31649109
- AI Meets Exascale Computing: Advancing Cancer Research With Large-Scale High Performance Computing
Bhattacharya T, Brettin T, Doroshow JH, Evrard YA, Greenspan EJ, Gryshuk AL, Hoang TT, Lauzon CBV, Nissley D*, Penberthy L, Stahlberg E, Stevens R, Streitz F, Tourassi G, Xia F, Zaki G
Front Oncol 2019
PubMed PMID: 31632915
- RIT1 oncoproteins escape LZTR1-mediated proteolysis
Castel P, Cheng A, Cuevas-Navarro A, Everman DB, Papageorge AG, Simanshu DK*, Tankka A, Galeas J, Urisman A, McCormick F.
Science. 2019;363(6432):1226-30
PubMed PMID: 30872527
- KRAS G13D sensitivity to neurofibromin-mediated GTP hydrolysis
Rabara D*, Tran TH*, Dharmaiah S*, Stephens RM*, McCormick F*, Simanshu DK*, Holderfield M*
Proc Natl Acad Sci U S A 2019
PubMed PMID: 31611389
- Distinct Binding Preferences between Ras and Raf Family Members and the Impact on Oncogenic Ras Signaling
Terrell EM, Durrant DE, Ritt DA, Sealover NE, Sheffels E, Spencer-Smith R, Esposito D*, Zhou Y, Hancock JF, Kortum RL, Morrison DK
Mol Cell 2019
PubMed PMID: 31606273
- Genome Assembly and Annotation of the Trichoplusia ni Tni-FNL Insect Cell Line Enabled by Long-Read Technologies
Talsania K, Mehta M, Raley C, Kriga Y, Gowda S, Grose C*, Drew M*, Roberts V*, Cheng KT, Burkett S, Oeser S, Stephens R*, Soppet D*, Chen X, Kumar P, German O, Smirnova T, Hautman C, Shetty J, Tran B, Zhao Y, Esposito D*
Genes (Basel). 2019;10(2)
PubMed PMID: 30678108
- Second harmonic generation detection of Ras conformational changes and discovery of a small molecule binder
Donohue E, Khorsand S, Mercado G, Varney KM, Wilder PT, Yu W, MacKerell AD Jr, Alexander P*, Van QN*, Moree B, Stephen AG*, Weber DJ, Salafsky J, McCormick F*
Proc Natl Acad Sci U S A 2019
PubMed PMID: 31399543
- PIP2 Influences the Conformational Dynamics of Membrane-Bound KRAS4b
McLean MA, Stephen AG*, Sligar SG
Biochemistry 2019
PubMed PMID: 31339036
- Organization of Farnesylated, Carboxymethylated KRAS4B on Membranes
Barklis E, Stephen AG*, Staubus AO, Barklis RL, Alfadhli A
J Mol Biol 2019
PubMed PMID: 31330153
- Structures of N-terminally processed KRAS provide insight into the role of N-acetylation
Dharmaiah S*, Tran TH*, Messing S*, Agamasu C*, Gillette WK*, Yan W*, Waybright T*, Alexander P*, Esposito D*, Nissley DV*, McCormick F*, Stephen AG*, Simanshu DK*
Sci Rep 2019
PubMed PMID: 31324887
- Blockade of leukemia inhibitory factor as a therapeutic approach to KRAS driven pancreatic cancer
Wang MT, Fer N*, Galeas J, Collisson EA, Kim SE, Sharib J, McCormick F*
Nat Commun 2019
PubMed PMID: 31296870
- Membrane curvature sensing of the lipid-anchored K-Ras small GTPase
Liang H, Mu H, Jean-Francois F*, Lakshman B*, Sarkar-Banerjee S, Zhuang Y, Zeng Y, Gao W, Zaske AM, Nissley DV*, Gorfe AA, Zhao W, Zhou Y
Life Sci Alliance 2019
PubMed PMID: 31296567
- Intercellular Transfer of Oncogenic KRAS via Tunneling Nanotubes Introduces Intracellular Mutational Heterogeneity in Colon Cancer Cells
Desir S, Wong P, Turbyville T*, Chen D*, Shetty M, Clark C, Zhai E, Romin Y, Manova-Todorova K, Starr TK, Nissley DV, Steer CJ, Subramanian S, Lou E
Cancers (Basel) 2019
PubMed PMID: 31247990
- Production of Farnesylated and Methylated Proteins in an Engineered Insect Cell System
Gillette W*, Frank P*, Perkins S*, Drew M*, Grose C*, Esposito D*
Methods Mol Biol 2019
PubMed PMID: 31152410
- Feasibility of using NF1-GRD and AAV for gene replacement therapy in NF1-associated tumors
Bai RY, Esposito D*, Tam AJ, McCormick F*, Riggins GJ, Wade Clapp D, Staedtke V
Gene Ther 2019
PubMed PMID: 31127187
- New weapons to penetrate the armor: Novel reagents and assays developed at the NCI RAS Initiative to enable discovery of RAS therapeutics
Esposito D*, Stephen AG*, Turbyville TJ*, Holderfield M*
Semin Cancer Biol 2019
PubMed PMID: 29432816
- KRAS Prenylation Is Required for Bivalent Binding with Calmodulin in a Nucleotide-Independent Manner
Agamasu C*, Ghirlando R, Taylor T*, Messing S*, Tran TH*, Bindu L*, Tonelli M, Nissley DV*, McCormick F*, Stephen AG*
Biophys Journal 2019
PubMed PMID: 30846362
- Quantitative biophysical analysis defines key components modulating recruitment of the GTPase KRAS to the plasma membrane
Lakshman B*, Messing S*, Schmid EM, Clogston JD, Gillette WK*, Esposito D*, Kessing B*, Fletcher DA, Nissley DV*, McCormick F*, Stephen AG*, Jean-Francois FL*
J Biol Chem 2018
PubMed ID: 30559287
- GradientScanSurv-An exhaustive association test method for gene expression data with censored survival outcome
Yi M*, Zhu R, Stephens RM*
PloS One 2018
PubMed ID: 30517129
- Label-Free Time-Gated Luminescent Detection Method for the Nucleotides with Varying Phosphate Content
Kopra K, Seppälä T, Rabara D*, Abreu-Blanco M*, Kulmala S, HolderfieldM*, Härmä H
Sensors (Basel) 2018
PubMed ID: 30453509
- The bacterial Ras/Rap1 site-specific endopeptidase RRSP cleaves Ras through an atypical mechanism to disrupt Ras-ERK signaling
Biancucci M, Minasov G, Banerjee A, Herrera A, Woida PJ, Kieffer MB, Bindu L*, Abreu-Blanco M*, Anderson WF, Gaponenko V, Stephen AG*, Holderfield M*, Satchell KJF
Sci Signal 2018
PubMed ID: 30279169
- Production of authentic geranylgeranylated KRAS4b using an engineered baculovirus system
Procter L*, Grose C*, Esposito D*
Protein Expr Purif 2018
PubMed ID: 29936133
- Direct molecular dissection of tumor parenchyma from tumor stroma in tumor xenograft using mass spectrometry-based glycoproteomics
Ye X*, Luke BT, Wei BR, Kaczmarczyk JA, Loncarek J, Dwyer JE, Johann DJ, Saul RG, Nissley DV*, McCormick F*, Whiteley GR*, Blonder J*
Oncotarget 2018
PubMed ID: 29899869
- Molecular recognition of RAS/RAF complex at the membrane: Role of RAF cysteine-rich domain
Travers T, López CA, Van QN*, Neale C, Tonelli M, Stephen AG*, Gnanakaran S
Sci Rep 2018
PubMed ID: 29855542
- Precise characterization of KRAS4b proteoforms in human colorectal cells and tumors reveals mutation/modification cross-talk
Ntai I, Fornelli L, DeHart CJ, Hutton JE, Doubleday PF, LeDuc RD, van Nispen AJ, Fellers RT, Whiteley G*, Boja ES, Rodriguez H, Kelleher NL
Proc Natl Acad Sci USA 2018
PubMed ID: 29610327
- Differential Effector Engagement by Oncogenic KRAS
Yuan TL, Amzallag A, Bagni R*, Yi M*, Afghani S, Burgan W*, Fer N*, Strathern LA*, Powell K*, Smith B, Waters AM*, Drubin D, Thomson T, Liao R, Greninger P, Stein GT, Murchie E, Cortez E, Egan RK, Procter L*, Bess M, Cheng KT*, Lee CS, Lee LC, Fellmann C, Stephens R*, Luo J, Lowe SW, Benes CH, McCormick F*
Cell Rep 2018
PubMed ID: 29444439
- New weapons to penetrate the armor: Novel reagents and assays developed at the NCI RAS Initiative to enable discovery of RAS therapeutics
Esposito D*, Stephen AG*, Turbyville TJ*, Holderfield M*
Semin Cancer Biol. 2018
PubMed ID: 29432816
- Targeting RAS-driven human cancer cells with antibodies to upregulated and essential cell-surface proteins
Martinko AJ, Truillet C, Julien O, Diaz JE, Horlbeck MA, Whiteley G*, Blonder J*, Weissman JS, Bandyopadhyay S, Evans MJ, Wells JA
Elife 2018
PubMed ID: 29359686
- K-Ras4B Remains Monomeric on Membranes over a Wide Range of Surface Densities and Lipid Compositions
Chung JK, Lee YK, Denson JP*, Gillette WK*, Alvarez S, Stephen AG*, Groves JT
Biophys J. 2018
PubMed ID: 29320680
- Efforts to Develop KRAS Inhibitors
Holderfield M*
Cold Spring Harb Perspect Med 2018
PubMed ID: 29101115
- Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies
Waters AM*, Ozkan-Dagliyan I, Vaseva AV, Fer N*, Strathern LA*, Hobbs GA, Tessier-Cloutier B, Gillette WK*, Bagni R*, Whiteley GR*, Hartley JL*, McCormick F*, Cox AD, Houghton PJ, Huntsman DG, Philips MR, Der CJ
Sci Signal 2018
PubMed ID: 28951536
- RAS Proteins and Their Regulators in Human Disease
Simanshu DK*, Nissley DV*, McCormick F*
Cell 2017
PubMed ID: 28666118
- Tumor RAS Gene Expression Levels Are Influenced by the Mutational Status of RAS Genes and Both Upstream and Downstream RAS Pathway Genes
Stephens RM*, Yi M*, Kessing B*, Nissley DV*, McCormick F*
Cancer Inform 2017
PubMed ID: 28634423
- A KRAS GTPase K104Q Mutant Retains Downstream Signaling by Offsetting Defects in Regulation
Yin G, Kistler S, George SD, Kuhlmann N, Garvey L*, Huynh M, Bagni RK*, Lammers M, Der CJ, Campbell SL
J Biol Chem 2017
PubMed ID: 28154176
- RAS signaling: Divide and conquer
Holderfield M*, Morrison DK
Nat Chem Biol 2016
PubMed ID: 27984577
- Comparative proteomics of a model MCF10A-KRasG12V cell line reveals a distinct molecular signature of the KRasG12V cell surface
Ye X, Chan KC, Waters AM*, Bess M, Harned A, Wei BR, Loncarek J, Luke BT, Orsburn BC, Hollinger BD*, Stephens RM*, Bagni R*, Martinko A, Wells JA, Nissley DV*, McCormick F*, Whiteley G*, Blonder J*
Oncotarget 2016
PubMed ID: 27894102
- Inhibition of Ras/Raf/MEK/ERK Pathway Signaling by a Stress-Induced Phospho-Regulatory Circuit
Ritt DA, Abreu-Blanco MT*, Bindu L*, Durrant DE, Zhou M, Specht SI, Stephen AG*, Holderfield M*, Morrison DK
Mol Cell 2016
PubMed ID: 27889448
- Structural basis of recognition of farnesylated and methylated KRAS4b by PDEδ
Dharmaiah S*, Bindu L*, Tran TH*, Gillette WK*, Frank PH*, Ghirlando R, Nissley DV*, Esposito D*, McCormick F*, Stephen AG*, Simanshu DK*
Proc Natl Acad Sci USA 2016
PubMed ID: 27791178
- Single Synonymous Mutations in KRAS Cause Transformed Phenotypes in NIH3T3 Cells
Waters AM*, Bagni R*, Portugal F*, Hartley JL*
PLoS One 2016
PubMed ID: 27684555
- Farnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions
Gillette WK*, Esposito D*, Abreu Blanco M*, Alexander P*, Bindu L*, Bittner C*, Chertov O*, Frank PH*, Grose C*, Jones JE*, Meng Z*, Perkins S*, Van Q, Ghirlando R, Fivash M*, Nissley DV*, McCormick F*, Holderfield M*, Stephen AG*
Sci Rep 2015
PubMed ID: 26522388
- Progress in targeting RAF kinases for cancer therapy
Turbyville TJ*, Holderfield M*
Per Med 2015
PubMed ID: 29771643
- Dragging RAS back in the ring
Stephen AG*, Esposito D*, Bagni RK*, McCormick F*
Cancer Cell 2014
PubMed ID: 24651010