University of California San Francisco – 2

Integrative Genomic Approaches in Neuroblastoma (NBL)

The CTD² Center at University of California San Francisco (UCSF-2) used an integrative genomics approach to reveal unidentified mRNA splicing patterns in neuroblastoma.

Expression Analysis of Superior Cervical Ganglion from Backcrossed TH-MYCN Transgenic Mice

The CTD² Center at University of California San Francisco (UCSF-2) used genetic analysis of the peripheral sympathetic nervous system to identify potential therapeutic targets in neuroblastoma.

Gene Expression Profiling of Normal Mouse Skin, Hras WT and Hras -/-

This data set contains the transcriptional profiles of 20 dorsal skin samples from eight-week-old mice.

Mice were generated by crossing FVB/N to Mus spretus mice to generate F1 mice, and then crossing F1 mice back to the FVB/N strain. 10  FVB/N mice lacking Hras1 (aka HrasKO, Hras-/-) and 10 FVB/N mice with wild-type Hras1 were generated.

Human Neuroepithelial Stem Cells as a Model System for Medulloblastoma

This study demonstrates that neuroepithelial stem cells derived from human induced pluripotent stem cells could be a good experimental resource to evaluate genetic mutations and understand tumorigenesis in medulloblastoma.

Experimental Approaches

Human neuroepithelial stem (NES) cells were transduced with MYCN to evaluate the usage of NES cells as progenitors of the cerebellar primordium. Genomic analysis was performed to identify copy number changes and mutations. Raw data for whole exome sequencing, RNA-seq, and amplicon sequencing are stored in the European Genome Archive (EGA) with accession number EGAS00001003620.