HER2-CAR T cells and Chemotherapy in Treating Patients with Recurrent Brain or Leptomeningeal Metastases
Trial Status: closed to accrual
This phase I trial studies the side effects and best dose of HER2-CAR T cells alone or with chemotherapy in treating patients with cancer that has spread to the brain (metastasized) or leptomeninges and has come back (recurrent). HER2-CAR T cells delivered into the ventricles of the brain may recognize and kill tumor cells. Chemotherapy drugs, such as cyclophosphamide and fludarabine, work in different ways to stop the growth of tumor cells, either by killing the cells, by stopping them from dividing, or by stopping them from spreading. Giving chemotherapy with HER2-CAR T cells may kill more tumor cells.
Inclusion Criteria
- SCREENING: Participant has treated brain and/or leptomeningeal metastases that has not recurred OR * Such participants are eligible to enroll in the study and undergo leukapheresis, but they cannot start treatment with HER2-CAR T cells until there is evidence of tumor progression/recurrence
- SCREENING: Participant has recurrent brain metastases after radiation therapy OR
- SCREENING: Participant has recurrent leptomeningeal metastases after intrathecal chemotherapy OR
- SCREENING: Participant has untreated brain or leptomeningeal metastases and refuses to undergo radiation and/or intrathecal chemotherapy * Note: Participants with leptomeningeal metastasis (diagnosed by positive CSF cytology or characteristic findings on brain or spine magnetic resonance imaging [MRI]) may have concomitant brain metastases, but having both is not required
- SCREENING: Participant must have a Karnofsky performance status (KPS) >= 70
- SCREENING: Participant must have a life expectancy of >= 8 weeks
- SCREENING: The effects of HER2-CAR T cells on a developing fetus are unknown. For this reason, women of child-bearing potential must have negative serum pregnancy test and agree to use a reliable form of birth control prior to study entry and for at least two months following duration of study participation. Male research participants must agree to use a reliable form of birth control and not donate sperm during the study and for at least six months afterwards
- SCREENING: Participant has a histologically confirmed cancer which is HER2+, defined as 3+ by immunohistochemistry (IHC) or gene amplification by fluorescence in situ hybridization (FISH)
- SCREENING: Participant must have the ability to understand and the willingness to sign a written informed consent
- ELIGIBILITY TO PROCEED WITH LEUKAPHERESIS: At least 2 weeks must have elapsed since the participant received his/her last dose of prior targeted agents, chemotherapy or radiation; at the principal investigator (PI)'s discretion, exception can be made for investigational agents that are delivered locally into the CSF
- ELIGIBILITY TO PROCEED WITH LEUKAPHERESIS: Participant must be willing to undergo placement of a catheter for central venous access if s/he does not have adequate peripheral venous access for collection of T cells
- ELIGIBILITY TO PROCEED WITH RICKHAM RESERVOIR PLACEMENT: Participant has signed main treatment consent
- ELIGIBILITY TO PROCEED WITH RICKHAM RESERVOIR PLACEMENT: Creatinine < 1.6 mg/dL
- ELIGIBILITY TO PROCEED WITH RICKHAM RESERVOIR PLACEMENT: White blood cell (WBC) > 2,000/uL (or absolute neutrophil count [ANC] > 1,000)
- ELIGIBILITY TO PROCEED WITH RICKHAM RESERVOIR PLACEMENT: Platelets >= 100,000/uL
- ELIGIBILITY TO PROCEED WITH RICKHAM RESERVOIR PLACEMENT: International normalized ratio (INR) must be < 1.3
- ELIGIBILITY TO PROCEED WITH RICKHAM RESERVOIR PLACEMENT: Bilirubin < 1.5 mg/dL
- ELIGIBILITY TO PROCEED WITH RICKHAM RESERVOIR PLACEMENT: Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) < 2.5 X upper limits of normal
- ELIGIBILITY TO PROCEED WITH RICKHAM RESERVOIR PLACEMENT: Platelet count needs to be > 50,000/uL (platelet transfusions are allowed to get platelet count > 50,000/uL)
- ELIGIBILITY TO PROCEED WITH LYMPHODEPLETION (TREATMENT ARMS 4 AND 5 ONLY): Lymphodepletion will be administered in the outpatient setting no sooner than one week after Rickham placement and may be given 3 to 14 days prior to the HER2 CAR T cell infusion based on PI discretion and research participant’s eligibility
- ELIGIBILITY TO PROCEED WITH LYMPHODEPLETION (TREATMENT ARMS 4 AND 5 ONLY): Participant must be taking =< 6 mg/ day of dexamethasone (or the equivalent dose of another corticosteroid) during lymphodepletion and CAR T cell therapy
- ELIGIBILITY TO PROCEED WITH LYMPHODEPLETION (TREATMENT ARMS 4 AND 5 ONLY): Participant agrees to stop treatment with chemotherapy or endocrine therapy during lymphodepletion (if applicable) and the first 3 cycles of the HER2-CAR T cell study
- ELIGIBILITY TO PROCEED WITH LYMPHODEPLETION (TREATMENT ARMS 4 AND 5 ONLY): Participant has adequate bone marrow function as evidenced by platelets > 50,000/uL. However, lymphodepletion may proceed after platelet transfusion is given, and the post transfusion platelet count is >= 50,000/uL
- ELIGIBILITY TO PROCEED WITH LYMPHODEPLETION (TREATMENT ARMS 4 AND 5 ONLY): Serum creatinine < 1.8 mg/dL
- ELIGIBILITY TO PROCEED WITH LYMPHODEPLETION (TREATMENT ARMS 4 AND 5 ONLY): If the research participant has new symptoms of congestive heart failure (CHF), cardiomyopathy, myocarditis, myocardial infarction (MI), or exposure to cardiotoxic medications, they already had a cardiac consultation, creatinine phosphokinase (CPK), and troponin testing at pre study deeming them fit for study participation
- ELIGIBILITY TO PROCEED WITH CAR T CELL INFUSION (WITH OR WITHOUT LYMPHODEPLETION): Participant must be taking =< 6 mg/ day of dexamethasone (or the equivalent dose of another corticosteroid) during CAR T cell therapy
- ELIGIBILITY TO PROCEED WITH CAR T CELL INFUSION (WITH OR WITHOUT LYMPHODEPLETION): Participants agrees to stop treatment with chemotherapy or endocrine therapy during the first 3 cycles of the HER2-CAR T cell study
- ELIGIBILITY TO PROCEED WITH CAR T CELL INFUSION (WITH OR WITHOUT LYMPHODEPLETION): Platelets > 50,000/uL. However, HER2 CAR T cell infusion may proceed after platelet transfusion is given, and the post transfusion platelet count is >= 50,000/uL
- ELIGIBILITY TO PROCEED WITH CAR T CELL INFUSION (WITH OR WITHOUT LYMPHODEPLETION): Serum creatinine < 1.8 mg/dL
- ELIGIBILITY TO PROCEED WITH CAR T CELL INFUSION (WITH OR WITHOUT LYMPHODEPLETION): If the research participant has new symptoms of congestive heart failure (CHF), cardiomyopathy, myocarditis, MI, or exposure to cardiotoxic medications, they already had a cardiac consultation, creatinine phosphokinase (CPK), and troponin testing at pre study deeming them fit for study participation
- ELIGIBILITY TO PROCEED WITH CAR T CELL INFUSION (WITH OR WITHOUT LYMPHODEPLETION): Participant has a released cryopreserved HER2-CAR T cell product
- ADDITIONAL ELIGIBILITY TO START CYCLE 1: Participant must have at least 1 metastatic brain lesion (measurable disease per RANO-Brain Metastases [BM] is not required—i.e., participant does not need to have at least 1 metastatic lesion >= 1 cm in longest dimension) that is new or increasing in size, or CSF cytology and/or radiographic findings consistent with leptomeningeal metastases
- ADDITIONAL ELIGIBILITY TO START CYCLE 1: Participants whose brain metastases have been treated with whole brain radiation (WBRT) or stereotactic radiosurgery (SRS): previously radiated metastases must have >= 20% increase in longest diameter that is also >= 5 mm absolute increase from prior brain MRI or histopathologically proven recurrent tumor. Otherwise, new or not previously radiated lesions must be present * Note: Participants who have undergone local therapy (surgical resection or biopsy, or SRS), must have recovered from all acute side effects before starting treatment with HER2-CAR T cells
- ADDITIONAL ELIGIBILITY TO START CYCLE 1: If a participant with brain metastases does not meet the above criteria, but there is still concern by the study team that changes in an enhancing brain lesion seen on brain MRI could be due to tumor progression, then the participant can undergo resection or biopsy of the lesion(s) to distinguish between tumor progression versus radiation necrosis. If there is histopathological evidence of tumor progression, then the participant can proceed with study treatment
- ADDITIONAL ELIGIBILITY TO START CYCLE 1: Participants must have recovered from toxicity (=< grade 1) of prior therapy (excluding alopecia and peripheral neuropathy)
- ADDITIONAL ELIGIBILITY TO START CYCLE 1: Wash-out requirements (standard or investigational). The required waiting period between the last dose of the most recent chemotherapy agent(s) and first dose of HER2-CAR T cells is: * Four weeks for a cytotoxic chemotherapy, except for capecitabine, which will only require a 1 week waiting period from the last dose (on a dosing schedule of twice daily [bid] x 14 days, then off x 7 days); * At least 6 weeks must have passed since the completion of a nitrosourea-containing chemotherapy regimen; * At least four half-lives for a targeted agent
Exclusion Criteria
- EXCLUSION CRITERIA FOR STUDY SCREENING: Research participant requires supplemental oxygen to keep saturation greater than 95% and the situation is not expected to resolve within 2 weeks
- EXCLUSION CRITERIA FOR STUDY SCREENING: Research participants with a known history of congestive heart failure (CHF) or cardiac symptoms consistent with NYHA classification III-IV within 6 months prior to day 1 of protocol treatment, cardiomyopathy, myocarditis, myocardial infarction (MI), exposure to cardiotoxic medications or with clinical history suggestive of the above must have an EKG and echocardiogram (ECHO) performed within 42 days prior to registration and as clinically indicated while on treatment
- EXCLUSION CRITERIA FOR STUDY SCREENING: Research participants with new symptoms of CHF, cardiomyopathy, myocarditis, MI, or exposure to cardiotoxic medications must have a cardiac consultation, creatinine phosphokinase (CPK), and troponin testing at pre study and as clinically indicated
- EXCLUSION CRITERIA FOR STUDY SCREENING: Research participant requires dialysis
- EXCLUSION CRITERIA FOR STUDY SCREENING: Research participant has uncontrolled seizure activity and/or clinically evident progressive encephalopathy
- EXCLUSION CRITERIA FOR STUDY SCREENING: Failure of research participant to understand the basic elements of the protocol and/or the risks/benefits of participating in this phase 1 study. A legal guardian may substitute for the research participant
- EXCLUSION CRITERIA FOR STUDY SCREENING: Participant is unwilling to stop treatment with chemotherapy or endocrine therapy during the first 3 cycles of the HER2-CAR T cell study
- EXCLUSION CRITERIA FOR STUDY SCREENING: Participant has a coagulopathy or bleeding disorder or cannot safely discontinue anticoagulation prior to placement of a Rickham reservoir
- EXCLUSION CRITERIA FOR STUDY SCREENING: Participant has a chronic or active viral infection of the CNS
- EXCLUSION CRITERIA FOR STUDY SCREENING: Participant has any uncontrolled illness, including ongoing or active infection; participant has known active hepatitis B or C infection; participants with any signs or symptoms of active infection, positive blood cultures or radiological evidence of infections
- EXCLUSION CRITERIA FOR STUDY SCREENING: Participant is human immunodeficiency virus (HIV) seropositive based on testing performed within 4 weeks of screening
- EXCLUSION CRITERIA FOR STUDY SCREENING: Participant has an autoimmune disease
- EXCLUSION CRITERIA FOR STUDY SCREENING: Participant has another active malignancy
- EXCLUSION CRITERIA FOR STUDY SCREENING: Participants with lung metastases (exception may be allowed per PI discretion for participants that are not symptomatic from their lung metastases)
- EXCLUSION CRITERIA FOR STUDY SCREENING: Participant is unable to undergo a brain MRI
- EXCLUSION CRITERIA FOR STUDY SCREENING: Participant is breast feeding. Because there is an unknown but potential risk for adverse events in nursing infants secondary to treatment of the mother with HER2-CAR T cells, breastfeeding should be discontinued if the mother wants to participate in this study
- EXCLUSION CRITERIA FOR STUDY SCREENING: Patient has a serious medical or psychiatric illness that could, in the investigator’s opinion, potentially interfere with the safety monitoring requirements and completion of treatment according to this protocol
- ELIGIBILITY TO PROCEED WITH LEUKAPHERESIS: Research participant must not require more than 6 mg/day of dexamethasone (or the equivalent dose of another corticosteroid) on the day of leukapheresis
- ELIGIBILITY TO PROCEED WITH LYMPHODEPLETION (TREATMENT ARMS 4 AND 5 ONLY): Participant does not have evidence of active infection and does not have a fever exceeding 38.5 degree Celsius; there is an absence of positive blood culture for bacteria, fungus, or virus within 48-hours prior to start of lymphodepletion and/or there aren’t any indications of meningitis
- ELIGIBILITY TO PROCEED WITH LYMPHODEPLETION (TREATMENT ARMS 4 AND 5 ONLY): Research participant’s serum total bilirubin or transaminases does not exceed 2 X upper limits of normal
- ELIGIBILITY TO PROCEED WITH LYMPHODEPLETION (TREATMENT ARMS 4 AND 5 ONLY): Research participant does not require supplemental oxygen to keep saturation greater than 95% and/or does not have presence of any radiographic abnormalities on chest x-ray that are positive
- ELIGIBILITY TO PROCEED WITH LYMPHODEPLETION (TREATMENT ARMS 4 AND 5 ONLY): Research participant does NOT have any known history of congestive heart failure (CHF) or cardiac symptoms consistent with NYHA classification III-IV within 6 months prior to Day 1 of protocol treatment (i.e. lymphodepletion), cardiomyopathy, myocarditis, Myocardial Infarction (MI), exposure to cardiotoxic medications or with clinical history suggestive of the above must have an EKG and echocardiogram (ECHO) performed within 42 days prior to registration and as clinically indicated while on treatment
- ELIGIBILITY TO PROCEED WITH CAR T CELL INFUSION (WITH OR WITHOUT LYMPHODEPLETION): Participant does not have evidence of active infection and does not have a fever exceeding 38.5 degrees C; there is an absence of positive blood culture for bacteria, fungus, or virus within 48-hours prior to HER2-CAR T cell infusion and/or there aren’t any indications of meningitis
- ELIGIBILITY TO PROCEED WITH CAR T CELL INFUSION (WITH OR WITHOUT LYMPHODEPLETION): Serum total bilirubin or transaminases does not exceed 2 x upper limits of normal
- ELIGIBILITY TO PROCEED WITH CAR T CELL INFUSION (WITH OR WITHOUT LYMPHODEPLETION): Research participant does not require supplemental oxygen to keep saturation greater than 95% and/or does not have presence of any radiographic abnormalities on chest x-ray that are positive
- ELIGIBILITY TO PROCEED WITH CAR T CELL INFUSION (WITH OR WITHOUT LYMPHODEPLETION): Research participant does NOT have any known history of congestive heart failure (CHF) or cardiac symptoms consistent with NYHA classification III-IV within 6 months prior to day 1 of protocol treatment, cardiomyopathy, myocarditis, myocardial infarction (MI), exposure to cardiotoxic medications or with clinical history suggestive of the above must have an EKG and echocardiogram (ECHO) performed within 42 days prior to registration and as clinically indicated while on treatment
- ADDITIONAL ELIGIBILITY TO START CYCLE 1: Research participant does not have uncontrolled seizure following surgery prior to starting the first CAR T cell dose
Study sponsor and potential other locations can be found on ClinicalTrials.gov for NCT03696030.
PRIMARY OBJECTIVES:
I. To assess the feasibility and safety of intraventricularly administered memory-enriched autologous HER2(EQ)BBzeta/CD19t+ T cells (HER2-chimeric antigen receptor [CAR] T cells) - either HER2(EQ)BBzeta/CD19t+ TCM in Arm 1, or HER2(EQ)BBzeta/CD19t+ TN/MEM in Arm 2 - either alone or following lymphodepletion, in participants with brain and/or leptomeningeal metastases from HER2 positive cancers.
II. To determine the maximum tolerated dose (MTD) and a recommended phase 2 dosing (RP2D) plan for Arm 2, alone or following lymphodepletion.
SECONDARY OBJECTIVES:
I. Assess cerebrospinal fluid (CSF) and peripheral blood for HER2-CAR T cell persistence and endogenous immune system activation.
II. Describe changes in cytokine levels in the CSF and peripheral blood.
III. Describe changes in circulating tumor cells in the CSF.
IV. In study participants who complete at least the first three cycles of HER2-CAR T cell infusions:
IVa. Describe the central nervous system (CNS) clinical benefit defined as disease response rate based on Response Assessment in Neuro-Oncology Criteria (RANO) criteria (stable disease [SD], partial response [PR], or complete response [CR] in the brain).
IVb. Describe the systemic clinical benefit based on Response Evaluation Criteria in Solid Tumors (RECIST) criteria.
IVc. Estimate the median central nervous system (CNS) progression-free and overall survival rates (mPFS and mOS), (newly diagnosed versus recurrent metastases).
V. In study participants who undergo tumor resection or biopsy during or after study treatment or upon autopsy, evaluate the tumor micro-environment for:
Va. HER2-CAR T cell persistence.
Vb. Immune cell subsets.
Vc. Cytokine levels.
Vd. HER2 antigen expression levels.
VI. Use biomathematical modeling of tumor growth to evaluate benefit of treatment.
OUTLINE: This is a dose-escalation study. Patients are assigned to 1 of 2 arms.
ARM 1: Patients receive HER2-CAR T cells via intraventricular administration over 5 minutes once weekly for 3 doses in the absence of disease progression or unacceptable toxicity. If patients continue to meet all eligibility criteria, they may receive additional cycles of HER2-CAR T cells at principal investigator's discretion.
ARM 2: Patients undergo lymphodepletion consisting of cyclophosphamide intravenously (IV) over 1 hour on days -5 to -3 and fludarabine IV 30 minutes on days -5 to -3. Within 3-14 days after, patients receive HER2-CAR T cells via intraventricular administration over 5 minutes once weekly for 3 doses in the absence of disease progression or unacceptable toxicity. If patients continue to meet all eligibility criteria, they may receive additional cycles of HER2-CAR T cells at principal investigator's discretion.
After completion of study treatment, participants are followed up at 4 weeks, 3, 6, 8, 10, and 12 months, and then for up to 15 years.
Trial PhasePhase I
Trial Typetreatment
Lead OrganizationCity of Hope Comprehensive Cancer Center
Principal InvestigatorJana L Portnow
- Primary ID17237
- Secondary IDsNCI-2018-01270
- ClinicalTrials.gov IDNCT03696030