CD19/CD22 Chimeric Antigen Receptor T Cells for the Treatment of B-Cell Acute Lymphoblastic Leukemia

Inclusion Criteria

• RECIPIENT INCLUSION CRITERIA:
• Disease status of B-cell ALL (one of the following criteria must apply) * Subjects in complete response (CR) must have a history of chemotherapy refractory disease defined as progression or stable disease after one line of chemotherapy, or relapsed disease after achieving prior CR OR must have other high risk ALL features including: CRLF2 rearrangement, Ph-like phenotype, MLL/KMT2a rearrangement, or hypodiploid karyotype * Subjects with persistent or relapsed minimal residual disease (MRD) (by flow cytometry, polymerase chain reaction [PCR], fluorescence in situ hybridization [FISH], or next generation sequencing) require verification of MRD in the peripheral blood or bone marrow on two occasions at least 2 weeks apart * Subjects with active ALL (defined as >= 5% bone marrow blasts, circulating blasts, or extramedullary disease) are eligible
• Age >= 18 and =< 65 years (i.e., from age 18 to < 66 years old) at the time of enrollment
• Eastern cooperative oncology group (ECOG) performance status of 0, 1, or 2; or Karnofsky >= 60%
• CD19 expression is required any time since diagnosis. CD19 expression may be detected by immunohistochemistry or by flow cytometry. The choice of whether to use flow cytometry or immunohistochemistry will be determined by what is the most easily available tissue sample in each subject. In general, immunohistochemistry will be used for lymph node biopsies, flow cytometry will be used for peripheral blood and bone marrow samples. Patients receiving prior CD19 CAR T cell or blinatumomab are eligible if there is no documented history of CD19 negativity on the malignant cells
• Subjects must have an human leukocyte antigen (HLA) matched related donor willing to undergo unstimulated apheresis for T cell collection for CAR T cell generation followed by granulocyte colony stimulating factor [GCSF] mobilized apheresis for Treg CD34+ HSPC infusion (Orca-T)
• Matched related donor who is an 8/8 match for HLA-A, -B, -C, and -DRB1, all typed using deoxyribonucleic acid (DNA)-based high-resolution methods
• Cardiac ejection fraction at rest >= 45%
• Total bilirubin < 2 times upper limit of normal (ULN) (patients with Gilbert’s syndrome may be included where hemolysis has been excluded and with approval of the medical monitor)
• Aspartate aminotransferase (AST)/alanine aminotransferase (ALT) =< 3 time ULN
• Calculated creatinine clearance >= 50 mL/min or creatinine < 2.0 mg/dL
• Diffusing capacity of the lung for carbon monoxide (DLCO) (adjusted for hemoglobin) >= 50%
• Subjects with central nervous system (CNS) involvement are eligible as long as there are no overt signs or symptoms that in the evaluation of the investigator would mask or interfere with the neurological assessment of toxicity
• Negative serum or urine beta-human chorionic gonadotropin (HCG) test in females of childbearing potential within 3 weeks of registration
• Subjects of childbearing or child fathering potential must be willing to practice birth control from the time of enrollment on this study and for four (4) months after receiving the preparative conditioning regimen
• Must be able to give informed consent. Legal authorized representative (LAR) is permitted if subject is cognitively able to provide verbal assent
• DONOR INCLUSION CRITERIA:
• Age >= 16 and =< 75 years at time of enrollment
• Karnofsky performance status of >= 70% defined by institutional standards
• Willing to donate for two separate apheresis collections (T cells and PBSC)
• HLA match to the patient in either one of the following classifications: * Matched related donor who is an 8/8 match for HLA-A, -B, -C, and -DRB1, all typed using DNA-based high-resolution methods
• Seronegative for HIV-1 ribonucleic acid (RNA) PCR; HIV 1 and HIV 2 Ab (antibody); HTLV-1 and HTLV-2 Ab; PCR+ or sAg negative (surface antigen for hepatitis B); negative for the Treponema pallidum antibody Syphilis screen; and negative for HIV-1 and hepatitis C by nucleic acid testing (NAT) within 40 days of donor apheresis procedures
• In the case that T pallidum antibody tests are positive, donors must: * Be evaluated and show no evidence of syphilis infection of any stage by physical exam and history * Have completed effective antibiotic therapy to treat syphilis * Have a documented negative non-treponemal test (such as rapid plasma reagin [RPR]) or in the case of a positive non-treponemal test must be evaluated by an infectious disease expert to evaluate for alternative causes of test positivity and confirm no evidence of active syphilitic disease
• Potential for Zika virus infection as defined as any of the following: * Medical diagnosis of Zika virus infection in the past 6 months * Residence in, or travel to, an area with active Zika virus transmission within the past 6 months. * Unprotected sex within the past 6 months with a person who is known to have either of the risk factors listed above
• Donors determined to be ineligible based on the results of Zika virus screening may be determined to be eligible if: * The donor has no signs or symptoms consistent with active Zika virus infection and * Either of the following is true: ** The donor is a first-degree or second-degree blood relative of the recipient ** Urgent medical need, meaning no comparable human cell product is available and the recipient is likely to suffer death or serious morbidity without the human cell product, as attested by the Investigator
• Ability to understand and the willingness to personally sign the written institutional review board (IRB)-approved informed consent document

Exclusion Criteria

• RECIPIENT EXCLUSION CRITERIA:
• History of other malignancy, unless disease free for at least 3 years. At the discretion of the principal investigator, subjects in remission for 1-2 years prior to enrollment may be deemed eligible after considering the nature of other malignancy, likelihood of recurrence for one year following therapy, and impact of prior treatment on risk of CD19/CD22-CAR T cells and Orca-T infusion. Subjects in remission < 1 year are not eligible. The following exceptions apply: * Nonmelanoma skin cancer or carcinoma in situ (e.g., cervix, bladder, breast) is eligible * Hormonal therapy in subjects in remission > 1 year will be allowed
• Patients who have undergone a prior allogeneic or autologous stem cell transplant
• Recipient positive anti-donor HLA antibodies against a mismatched allele in the selected donor determined by either: * A positive crossmatch test of any titer (by complement-dependent cytotoxicity or flow cytometric testing), or * The presence of anti-donor HLA antibody to any of the following HLA loci: HLA-A, -B, -C, –DRB1, -DQB1, -DQA1, -DPB1, or -DPA1, with mean fluorescence intensity (MFI) >1000 by solid phase immunoassay
• Presence of fungal, bacterial, viral, or other infection that is uncontrolled. Simple urinary tract infection (UTI) and uncomplicated bacterial pharyngitis are permitted if responding to active treatment
• Known history of infection with any of the following: * Human immunodeficiency virus (HIV) * Hepatitis B (hepatitis B virus surface antigen [HBsAg] positive)** * Hepatitis C virus (anti hepatitis C virus [HCV] positive)** ** A history of hepatitis B or hepatitis C is permitted if the viral load is undetectable per quantitative PCR and/or nucleic acid testing
• Currently receiving corticosteroids or other immunosuppressive therapy. Topical corticosteroids or oral systemic corticosteroid doses less than or equal to 10 mg/day are allowed
• Planned pharmaceutical in vivo or ex vivo T cell depletion, e.g., post-transplant cyclophosphamide (Cy), peri-transplant anti-thymocyte globulin (ATG), or alemtuzumab. For patients that have previously been exposed to a T cell-depleting agent, a 5-half-life washout of the agent must occur prior to planned transplant day 0
• Hyperleukocytosis (>= 50,000 blasts/uL) or rapidly progressive disease that in the estimation of the investigator and sponsor would compromise ability to complete study therapy
• History of myocardial infarction, cardiac angioplasty or stenting, unstable angina, or other clinically significant cardiac disease within 12 months of enrollment
• Pregnant or breast feeding
• Patients with known autoimmune disease requiring the use of systemic immunosuppressive therapy within the last year
• Presence of a seizure disorder, cerebrovascular ischemia/hemorrhage, dementia, cerebellar disease, or any autoimmune disease with CNS involvement that in the judgment of the investigator may impair the ability to evaluate neurotoxicity
• Any medical condition that in the judgement of the investigator is likely to interfere with assessment of safety or efficacy of study treatment
• DONOR EXCLUSION CRITERIA:
• Evidence of active infection
• Seropositive for HIV-1 or -2, HTLV-1 or -2
• Positive serologic or PCR test results indicating acute or chronic HBV infection: Donors whose HBV infection status cannot be determined conclusively by serologic test results must be negative for HBV by PCR to be eligible for study participation
• Pregnant or breastfeeding female
• Medical, physical, or psychological reason that would place the donor at increased risk for complications from growth factor or leukapheresis