Genetically Engineered Cells (PSCA ɣδ-Enriched CAR T-cells) for the Treatment of Castration Resistant Prostate Cancer Metastatic in the Bone

Inclusion Criteria

• Men with metastatic castration-resistant prostate cancer (CRPC) to the bone with evidence of imaging progression based on the Prostate Cancer Clinical Trials Working Group 3 (PCWG3) criteria
• Prior therapies with at least one line of chemotherapy and one new androgen receptor targeted therapy (abiraterone, enzalutamide, apalutamide, or darolutamide).
• For patients who are on zoledronic acid a booster dose of zoledronic acid is required if the last dose of zoledronic acid is > 4 weeks prior to lymphodepletion chemo. If a patient is receiving denosumab, the next dose of denosumab needs to be changed to zoledronic acid and he needs to receive at least 1 dose of zoledronic acid prior to lymphodepletion chemotherapy. If a patient is not on zoledronic acid or denosumab, he needs to receive at least 2 doses of every 4 weeks of zoledronic acid prior to lymphodepletion chemotherapy. Zoledronic acid is recommended not to be resumed prior to week 8. After week 8, the resumption of zoledronic acid and the subsequent zoledronic acid treatment will be at the discretion of the treating physician
• No anticancer therapy (chemotherapy, biologic therapy, radiation or immunotherapy) in the 3 weeks before the T cell infusion (and all hematologic effects have resolved). No prior treatment with Radium 223 or Puvicto within 3 months of T cell infusion. No prior immunotherapy with checkpoint blockade (e.g., PD-1 inhibitor, PDL1 inhibitor, or CTL4- antagonist or similar agent) in the 6 months before the T cell infusion (and all clinically significant related side effects must be resolved)
• Males ≥ 18 years of age
• Eastern Cooperative Oncology Group (ECOG) performance status ≤ 2 (or Karnofsky performance status ≥ 70%)
• Absolute neutrophil count > 1,200/mcL
• Platelets > 100,000/mcL
• Hemoglobin > 9 g/dL
• Total bilirubin < 1.5 x institutional upper limit of normal (ULN)
• Aspartate aminotransferase (AST) (serum glutamic-oxaloacetic transaminase [SGOT])/alanine aminotransferase (ALT) (serum glutamic-pyruvic transaminase [SGPT]) < 2.5 × institutional ULN
• Creatinine clearance > 40 mL/min
• Amylase and lipase < 1.5 x institutional upper
• Left ventricular ejection fraction ≥ 50% left ventricular ejection fraction assessment must have been performed within 8 weeks of enrollment
• Life expectancy of at least 6 months
• The effects of CAR T cell infusion on the developing human fetus are unknown. Although patients who are eligible for this study will not have childbearing potential, any patient the treating doctor or investigator deems to have child fathering potential must agree to use adequate contraception from the time of screening to at least 6 months after administration of gamma delta enriched T cell infusion. Any female partner(s) with childbearing potential, of these participants, should also use adequate contraception during the same time-period
• Ability to understand and the willingness to sign a written informed consent document

Exclusion Criteria

• Known active hepatitis B infection, known history of hepatitis C or HIV infection
• Known dental issues like osteonecrosis of jaw that excludes the use of zoledronic acid
• Any of the following cardiac conditions: * Clinically significant heart disease (New York Heart Association class 3 or 4) or symptomatic congestive heart failure * Myocardial infarction < 6 months before enrollment * History of clinically significant ventricular arrhythmia or unexplained syncope that is not believed to be vasovagal in nature or due to dehydration * History of severe non-ischemic cardiomyopathy with ejection fraction < 20% * Findings on baseline electrocardiogram (ECG) or ECHO that, in the opinion of the patient’s treating physician or investigator, would require medical intervention before anticancer therapy
• Active autoimmune disease (excluding autoimmune thyroid disease on a stable thyroid regimen). Such conditions include but are not limited to systemic lupus erythematous, rheumatoid arthritis, ulcerative colitis, Crohn’s disease, and temporal arteritis
• Known or suspected leptomeningeal disease and patients with metastases to the brain stem, midbrain, pons, or medulla
• Known or suspected untreated brain metastases. Patients with radiographically stable, asymptomatic previously irradiated lesions are eligible provided patient is > 4 weeks beyond completion of cranial irradiation and > 3 weeks off of corticosteroid therapy at the time of study intervention
• Prior history of clinically significant seizure disorder (e.g., not including childhood febrile seizures)
• Any concurrent active malignancies, defined as malignancies requiring any therapy other than expectant observation, because adverse events (AEs) resulting from these malignancies, or their treatment may confound our assessment of the safety of adoptive T cell therapy for ovarian cancer
• Any of the following within 28 days of first date of study treatment * Serious uncontrolled medical illness or disorder that in the opinion of the treating physician would make the patient ineligible for the study * Active uncontrolled infection (with the exception of uncomplicated urinary tract infection)
• Prior history of pancreatitis
• Any other issue which, in the opinion of the treating physician or principal investigator, would make the patient ineligible for the study