Intravesical Adoptive Cell Therapy (ACT) with Tumor Infiltrating Lymphocytes (TIL) for the Treatment of High Grade Non-Muscle Invasive Bladder Cancer that Is Exposed to Bacillus Calmette-Guerin (BCG)

Inclusion Criteria

• SCREENING INCLUSION CRITERIA: BCG exposed high grade (HG) NMIBC and healthy enough to participate
• Participants must have: * Histologically confirmed urothelial cell NMIBC (T1, Ta, and/or Tis) and ** Bladder tumors with variant histology or mixed histology can be enrolled if the urothelial component is greater than 50% of the transurethral resection (TUR) specimen * If Ta and T1, patients must have undergone complete restaging transurethral resection of bladder tumor (TURBT) to confirm absence of muscle invasion (T2), however residual carcinoma in situ (CIS) is acceptable ** This restaging can be considered the primary tumor harvest if patients have had a previous resection
• Have cytoscopic evidence of measurable disease. (There is no minimum measurement to be considered measurable disease. Any visible evidence is considered recurrence.)
• A tissue specimen may be obtained which is appropriate for TIL preparation. The tissue may be collected through a procedure the patient otherwise requires for treatment purposes. Alternatively, and in consultation with a surgical specialist, a separate procedure of limited risk to the patient (such as a repeat bladder biopsy) may be performed specifically for tissue collection purposes
• Age >= 18 year
• Eastern Cooperative Oncology Group (ECOG) performance status 0-1
• Absolute neutrophil count >= 1,000/mcL (performed within 7 days ([+ 3 day window] of enrollment)
• Platelets >= 100,000/mcL (performed within 7 days ([+ 3 day window] of enrollment)
• Total bilirubin =< 1.5 x institutional upper limit of normal (ULN) or =< 3 x ULN if confirmed Gilbert's syndrome (persistent or recurrent hyperbilirubinemia that is predominantly unconjugated in the absence of hemolysis or hepatic pathology) (performed within 7 days ([+ 3 day window] of enrollment)
• Aspartate aminotransferase (AST) (serum glutamic oxaloacetic transaminase [SGOT]) / alanine aminotransferase (ALT) (serum glutamic pyruvic transaminase [SGPT]) =< 2.5 x institutional ULN unless liver metastases are present, in which case it must be =< 5 x ULN (performed within 7 days ([+ 3 day window] of enrollment)
• Prothrombin time (PT) and partial thromboplastin time (PTT) =< 1.5 x institutional ULN (performed within 7 days ([+ 3 day window] of enrollment)
• Serum creatinine 1.5 =< institutional ULN (performed within 7 days ([+ 3 day window] of enrollment)
• Glomerular filtration rate (GFR) > 30 mL/min – GFR calculations will be based on the Chronic Kidney Disease Epidemiology Collaboration Formula (CKD-EPI), per National Kidney Foundations current recommendations (performed within 7 days ([+ 3 day window] of enrollment)
• Albumin >= 2.5 g/dL (performed within 7 days ([+ 3 day window] of enrollment)
• Hemoglobin >= 9.0 g/dL (performed within 7 days ([+ 3 day window] of enrollment)
• Ability to understand and the willingness to sign a written informed consent document

Exclusion Criteria

• Any previous treatment with intravesical chemotherapy within the previous 6 months
• Current or prior use of any immunosuppressive medications, such as corticosteroids, within 14 days before enrollment * Oral hydrocortisone, only for the purposes of a documented and confirmed adrenal insufficiency diagnosis, is permitted if =< 25 mg daily total dose * Inhaled, intranasal, or topical corticosteroids are permitted
• Current or prior use of anticancer therapy that has been shown to effect lymphocyte function before TIL collection
• Uncontrolled intercurrent illness including, but not limited to, symptomatic congestive heart failure, unstable angina pectoris, cardiac arrhythmia (other than stable atrial fibrillation)
• Patients known to be human immunodeficiency virus (HIV) positive, hepatitis B or C positive, or both rapid plasma reagin (RPR) and fluorescent treponemal antibody (FTA) positive. (Hepatitis B surface or core antibody alone is not indicative of hepatitis B virus [HBV] infection)
• Known history of previous tuberculosis
• Receipt of live attenuated vaccination within 30 days prior to first anticipated dose of TIL
• History of allogeneic organ transplant
• History of primary immunodeficiency
• Any condition that, in the opinion of the investigator, would interfere with evaluation of study treatment or interpretation of patient safety or study results
• Patients with active systemic infections requiring intravenous antibiotics within 1 week prior to enrollment
• Any unresolved toxicity ( > Common Terminology Criteria for Adverse Events [CTCAE] version [v] 5 grade 2) from previous anti-cancer therapy. Subjects with irreversible toxicity that is not reasonably expected to be exacerbated by the investigational product may be included (e.g., hearing loss, peripheral neuropathy)
• History of pneumonitis or drug-related inflammatory lung disease
• Active or prior documented autoimmune disease within the past 2 years. NOTE: Subjects with vitiligo, Grave’s disease, limited site eczema, or limited site plaque psoriasis not requiring systemic treatment (within the past 2 years), or other autoimmune conditions which are not expected to recur, are allowed after approval from the medical monitor or principal investigator (PI)
• Patients with other prior malignancies must have had a >= 2-year disease-free interval, except for: in situ carcinoma of the cervix, in situ ductal carcinoma of the breast, in situ prostate cancer, in situ bladder cancer. These must have been deemed stable and not expected to relapse. In addition, early stage skin cancers, including basal, squamous cell cutaneous carcinoma, and melanoma, are permitted if previously treated with curative intent and not expected to relapse
• Women who are pregnant or lactating
• The effects of adoptive cell therapy (ACT) with tumor-infiltrating lymphocytes (TIL) infusion on the developing human fetus are unknown. For this reason, women of child-bearing potential (WOCBP) and men must agree to use adequate contraception (hormonal or barrier method of birth control; abstinence) prior to study entry and for the duration of study participation and until 4 months after completion of study drug administration. Those who do not agree must be excluded. Should a woman become pregnant or suspect she is pregnant while she or her partner is participating in this study, she should inform her treating physician immediately. WOCBP are defined as premenopausal women capable of becoming pregnant
• Penicillin allergy (Penicillin is used in the manufacturing of the cellular therapy product and therefore patients with a documented penicillin allergy are excluded from the trial) * Patients with antibiotic allergies per se are not excluded; although the production of TIL for adoptive transfer includes antibiotics, extensive washing after harvest will minimize systemic exposure to antibiotics.