RAS Cell Lines
Cell lines expressing mutant RAS genes are essential tools for finding ways to intervene in RAS cancers. RAS Initiative scientists have devoted significant effort to engineering and characterizing these cell lines so they will be a reliable resource for themselves and the larger RAS community.
"RASless" Mouse Embryonic Fibroblasts
Professor Mariano Barbacid's lab constructed a mouse embryonic fibroblast (MEF) line in which the HRAS and NRAS genes have been deleted, and the KRAS gene has been flanked by loxP sites (Drosten, et al., 2010). Additionally, an inducible Cre recombinase was knocked into the genome, so that the MEFs could be rendered RASless upon addition of 4-hydroxytamoxifen. Such RASless MEFs become quiescent, but they can be rescued to resume proliferation by lentiviral transduction of wild type or mutant RAS genes, or of activated RAS pathway genes such as the V600E mutant of BRAF.
The cell lines below are available to the RAS community. Each line has been characterized for clonality; confirmation of endogenous KRAS gene removal; identification of transgene insertion site(s); growth characteristics including proliferation rates and doubling times; analysis of signaling pathways; response to tool compounds; and exome sequencing to exclude lines with mutations in onco-relevant genes.
- KRAS 4B G12D
- KRAS 4B G12C
- KRAS 4B G12V
- KRAS 4B G13D
- KRAS 4B Q61L
- KRAS 4B Q61R
- KRAS 4B wild type
- KRAS 4A wild type
- HRAS wild type
- NRAS wild type
- BRAF V600E
For more information, contact William Burgan at 301-846-1785 or burganwe@mail.nih.gov.
Cancer Cell Lines with Mutant KRAS Genes
The heterogeneity of human cancers is a major obstacle to developing therapies. One way of dealing with that heterogeneity is to test large numbers of patient-derived cell lines for common vulnerabilities. For one such study, RAS Initiative researchers constructed GFP-labeled derivatives of dozens of cell lines that express mutant KRAS.
To obtain each line contact the vendor listed. For more information on our experience, contact Dom Esposito at dom.esposito@nih.gov.
| Cell Line | Tissue | KRAS Mutant | Number of Mutant Alleles | Source |
|---|---|---|---|---|
| AsPC-1 | pancreas | G12D | 2 | ATCC |
| Calu-1 | lung | G12C | 2 | ATCC |
| Capan-1 | pancreas | G12V | 2 | ATCC |
| Capan-2 | pancreas | G12V | 1 | ATCC |
| CFPAC-1 | pancreas | G12V | 1 | ATCC |
| COLO 668 | lung | G12V | 2 | Sigma |
| COR-L23 | lung | G12V | 2 | Sigma |
| DAN-G | pancreas | G12V | 2 | CLS Germany |
| GP2d | large_intestine | G12D | 1 | Sigma |
| HCT 116 | large_intestine | G13D | 1 | NCI DCTD |
| HCT-15 | large_intestine | G13D | 1 | NCI DCTD |
| HPAF-II | pancreas | G12D | 1 | ATCC |
| HUP-T4 | pancreas | G12V | 1 | Sigma |
| KP-3 | pancreas | G12V | 2 | JCRB |
| LCLC-97TM1 | lung | G12V | 2 | CLS Germany |
| LoVo | large_intestine | G13D | 1 | NCI DCTD |
| LS 180 | large_intestine | G12D | 1 | ATCC |
| LS513 | large_intestine | G12D | 1 | ATCC |
| LU65 | lung | G12C | 2 | RIKEN |
| LU99 | lung | G12C | 1 | RIKEN |
| MIA PaCa-2 | pancreas | G12C | 2 | ATCC |
| NCI-H1373 | lung | G12C | 2 | ATCC |
| NCI-H1385 | lung | G12C | 1 | ATCC |
| NCI-H1792 | lung | G12C | 1 | ATCC |
| NCI-H1944 | lung | G13D | 1 | ATCC |
| NCI-H2030 | lung | G12C | 2 | ATCC |
| NCI-H2122 | lung | G12C | 2 | ATCC |
| NCI-H23 | lung | G12C | 1 | NCI DCTD |
| NCI-H2444 | lung | G12V | 2 | ATCC |
| NCI-H358 | lung | G12C | 1 | ATCC |
| NCI-H441 | lung | G12V | 1 | ATCC |
| NCI-H647 | lung | G13D | 2 | ATCC |
| NCI-H727 | lung | G12V | 1 | ATCC |
| NCI-H747 | large_intestine | G13D | 1 | ATCC |
| Panc 02.03 | pancreas | G12D | 1 | ATCC |
| Panc 03.27 | pancreas | G12V | 1 | ATCC |
| Panc 04.03 | pancreas | G12D | 1 | ATCC |
| Panc 08.13 | pancreas | G12D | 2 | ATCC |
| Panc 10.05 | pancreas | G12D | 1 | ATCC |
| PA-TU-8902 | pancreas | G12V | 1 | DSMZ Germany |
| PK-1 | pancreas | G12D | 1 | RIKEN |
| PK-45H | pancreas | G12D | 2 | RIKEN |
| PK-59 | pancreas | G12D | 1 | RIKEN |
| QGP-1 | pancreas | G12V | 1 | JCRB |
| RCM-1 | large_intestine | G12V | 2 | JCRB |
| RERF-LC-Ad2 | lung | G12V | 2 | JCRB |
| SHP-77 | lung | G12V | 2 | NCI DCTD |
| SK-CO-1 | large_intestine | G12V | 1 | ATCC |
| SK-LU-1 | lung | G12D | 1 | ATCC |
| SNU-407 | large_intestine | G12D | 1 | Korean Cell Line Bank |
| SNU-C2A | large_intestine | G12D | 1 | ATCC |
| SU.86.86 | pancreas | G12D | 2 | ATCC |
| SW 1573 | lung | G12C | 2 | ATCC |
| SW 1990 | pancreas | G12D | 2 | ATCC |
| SW 900 | lung | G12V | 1 | ATCC |
| SW1463 | large_intestine | G12C | 2 | ATCC |
| SW403 | large_intestine | G12V | 1 | ATCC |
| SW480 | large_intestine | G12V | 2 | ATCC |
| SW620 | large_intestine | G12V | 2 | ATCC |
| SW837 | large_intestine | G12C | 1 | ATCC |
| T3M-10 | lung | G12D | 1 | RIKEN |
| T84 | large_intestine | G13D | 1 | ATCC |
| YAPC | pancreas | G12V | 1 | DSMZ Germany |