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National CryoEM Facility Acknowledged User Publications

The National Cryo-Electron Microscopy Facility (NCEF), which began operation in May 2017, provides cancer researchers access to the latest cryo-EM technology for high resolution imaging. Publications that report macromolecular structures obtained using data collected at NCEF are listed below.

Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly 
Wang L, Wu D, Robinson CV, Wu H, Fu TM
Mol Cell. 2020
PubMed ID: 33065002 

An ER translocon for multi-pass membrane protein biogenesis
McGilvray PT, Anghel SA, Sundaram A, Zhong F, Trnka MJ, Fuller JR, Hu H, Burlingame AL, Keenan RJ
Elife. 2020 
PubMed ID: 32820719 

Structures of filamentous viruses infecting hyperthermophilic archaea explain DNA stabilization in extreme environments
Wang F, Baquero DP, Beltran LC, Su Z, Osinski T, Zheng W, Prangishvili D, Krupovic M, Egelman EH
Proc Natl Acad Sci USA. 2020
PubMed ID: 32759221

Structural mechanism of two gain-of-function cardiac and skeletal RyR mutations at an equivalent site by cryo-EM
Iyer KA, Hu Y, Nayak AR, Kurebayashi N, Murayama T, Samsó M
Sci Adv. 2020 
PubMed ID: 32832689 

Mechanisms of activation and desensitization of full-length glycine receptor in lipid nanodiscs
Kumar A, Basak S, Rao S, Gicheru Y, Mayer ML, Sansom MSP, Chakrapani S
Nat Commun. 2020
PubMed ID: 32719334

Structure-based design of prefusion-stabilized SARS-CoV-2 spikes
Hsieh CL, Goldsmith JA, Schaub JM, DiVenere AM, Kuo HC, Javanmardi K, Le KC, Wrapp D, Lee AG, Liu Y, Chou CW, Byrne PO, Hjorth CK, Johnson NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS
Science. 2020
PubMed ID: 32703906

The structures of two archaeal type IV pili illuminate evolutionary relationships
Wang F, Baquero DP, Su Z, Beltran LC, Prangishvili D, Krupovic M, Egelman EH
Nat Commun. 2020
PubMed ID: 32647180

Cryo-EM Structure of NPF-bound Human Arp2/3 Complex and Activation Mechanism
Zimmet A, Van Eeuwen T, Boczkowska M, Rebowski G, Murakami K, Dominguez R
Sci Adv. 2020 
PubMed ID: 32548263

Electromechanical coupling in the hyperpolarization-activated K+ channel KAT1
Clark MD, Contreras GF, Shen R, Perozo E
Nature. 2020
PubMed ID: 32461693

Alternative splicing controls teneurin-latrophilin interaction and synapse specificity by a shape-shifting mechanism
Li J, Xie Y, Cornelius S, Jiang X, Sando R, Kordon SP, Pan M, Leon K, Südhof TC, Zhao M, Araç D
Nat Commun. 2020
PubMed ID: 32358586

Selective PP2A Enhancement through Biased Heterotrimer Stabilization
Leonard D, Huang W, Izadmehr S, O'Connor CM, Wiredja DD, Wang Z, Zaware N, Chen Y, Schlatzer DM, Kiselar J, Vasireddi N, Schüchner S, Perl AL, Galsky MD, Xu W, Brautigan DL, Ogris E, Taylor DJ, Narla G
Cell. 2020
PubMed ID: 32315618

Cryo-electron Microscopy Structure of the Acinetobacter baumannii 70S Ribosome and Implications for New Antibiotic Development
Morgan CE, Huang W, Rudin SD, Taylor DJ, Kirby JE, Bonomo RA, Yu EW
MBio. 2020
PubMed ID: 31964740

Structural basis for COMPASS recognition of an H2B-ubiquitinated nucleosome
Worden EJ, Zhang X, Wolberger C
Elife. 2020
PubMed ID: 31922488

Molecular basis of force-from-lipids gating in the mechanosensitive channel MscS
Reddy B, Bavi N, Lu A, Park Y, Perozo E
Elife. 2019
PubMed ID: 31880537

High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway
Butan C, Lara-Tejero M, Li W, Liu J, Galán JE
Proc Natl Acad Sci U S A. 2019
PubMed ID: 31744874

Structure-based characterization of novel TRPV5 inhibitors
Hughes TE, Del Rosario JS, Kapoor A, Yazici AT, Yudin Y, Fluck EC, Filizola M, Rohacs T, Moiseenkova-Bell VY
Elife. 2019
PubMed ID: 31647410

A packing for A-form DNA in an icosahedral virus
Wang F, Liu Y, Su Z, Osinski T, de Oliveira GAP, Conway JF, Schouten S, Krupovic M, Prangishvili D, Egelman EH
Proc Natl Acad Sci U S A. 2019
PubMedID: 31636205

Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases
Kondo Y, Ognjenovic J, Banerjee S, Karandur D, Merk A, Kullhanek K, Wong K, Roose JP, Subramaniam S, Kuriyan J
Science 2019
PubMed ID: 31604311

Structure of the thermo-sensitive TRP channel TRP1 from the alga Chlamydomonas reinhardtii
McGoldrick LL, Singh AK, Demirkhanyan L, Lin TY, Casner RG, Zakharian E, Sobolevsky AI
Nat Commun. 2019
PubMed ID: 31519888

Cryo-Electron Microscopy Structure of an Acinetobacter baumannii Multidrug Efflux Pump
Su CC, Morgan CE, Kambakam S, Rajavel M, Scott H, Huang W, Emerson CC, Taylor DJ, Stewart PL, Bonomo RA, Yu EW
MBio. 2019 
PubMed ID: 31266873

Ambidextrous helical nanotubes from self-assembly of designed helical hairpin motifs
Hughes SA, Wang F, Wang S, Kreutzberger MAB, Osinski T, Orlova A, Wall JS, Zuo X, Egelman EH, Conticello VP
Proc Natl Acad Sci U S A. 2019
PubMed ID: 31262809

Regulatory switch at the cytoplasmic interface controls TRPV channel gating
Zubcevic L, Borschel WF, Hsu AL, Borgnia MJ, Lee SY
Elife. 2019
PubMed ID: 31070581

Mechanism of Cross-talk between H2B Ubiquitination and H3 Methylation by Dot1L
Worden EJ, Hoffman NA, Hicks CW, Wolberger C
Cell 2019
PubMed ID: 30765112

Cryo-EM structure of the NLRC4CARD filament provides insights into how symmetric and asymmetric supramolecular structures drive inflammasome assembly
Matyszewski M, Zheng W, Lueck J, Antiochos B, Egelman EH, Sohn J
J. Biol. Chem. 2018
PubMed ID: 30385506

Cryo-EM reveals two distinct serotonin-bound conformations of full-length 5-HT3A receptor
Basak S, Gicheru Y, Rao S, Sansom MSP, Chakrapani S
Nature 2018
PubMed ID: 30401837

Cryo-EM structures of ASC and NLRC4 CARD filaments reveal a unified mechanism of nucleation and activation of caspase-1
Li Y, Fu TM, Lu A, Witt K, Ruan J, Shen C, Wu H
Proc. Natl. Acad. Sci. USA 2018
PubMed ID: 30279182

Structural insights on TRPV5 gating by endogenous modulators
Hughes TET, Pumroy RA, Yazici AT, Kasimova MA, Fluck EC, Huynh KW, Samanta A, Molugu SK, Zhou ZH, Carnevale V, Rohacs T, Moiseenkova-Bell VY
Nat. Commun. 2018
PubMed ID: 30305626

Structure and gating mechanism of the transient receptor potential channel TRPV3
Singh AK, McGoldrick LL, Sobolevsky AI
Nat. Struct. Mol. Biol. 2018
PubMed ID: 30127359

Mechanism of calmodulin inactivation of the calcium-selective TRP channel TRPV6
Singh AK, McGoldrick LL, Twomey EC, Sobolevsky AI
Sci. Adv. 2018
PubMed ID: 30116787

DNA melting initiates the RAG catalytic pathway
Ru H, Mi W, Zhang P, Alt FW, Schatz DG, Liao M, Wu H
Nat. Struct. Mol. Biol. 2018
PubMed ID: 30061602

Cryo-EM structure of the gasdermin A3 membrane pore
Ruan J, Xia S, Liu X, Lieberman J, Wu H
Nature 2018
PubMed ID: 29695864

Cryo-EM structure of 5-HT3A receptor in its resting conformation
Basak S, Gicheru Y, Samanta A, Molugu SK, Huang W, Fuente M, Hughes T, Taylor DJ, Nieman MT, Moiseenkova-Bell V, Chakrapani S
Nat. Commun. 2018
PubMed ID: 29410406


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