Skip to main content

Publications from the RAS Initiative

The NCI RAS Initiative has a "hub-and-spokes" structure, in that its scientists work both independently and in collaboration with academic and commercial entities. The list of RAS Initiative publications below reflects the balance between the public responsibilities of the National Cancer Institute and the requirements of our external partners.

Authors who names are marked with an asterisk (*) are part of the RAS Initiative.

  • Classical RAS proteins are not essential for paradoxical ERK activation induced by RAF inhibitors
    Lai LP*, Fer N*, Burgan W*, Wall V*, Xu B*, Soppet D*, Esposito D*, Nissley DV*, McCormick F
    Proc Natl Acad Sci U S A 2022
    PubMed PMID: 35091470
  • Insights into the cross talk between effector and allosteric lobes of KRAS from methyl conformational dynamics
    Chao FA*, Dharmaiah S*, Taylor T*, Messing S*, Gillette W*, Esposito D*, Nissley DV*, McCormick F, Byrd RA, Simanshu DK*, Cornilescu G*
    J Am Chem Soc 2022
    PubMed PMID: 35213144
  • Machine learning-driven multiscale modeling reveals lipid-dependent dynamics of RAS signaling proteins
    Ingólfsson HI, Neale C, Carpenter TS, Shrestha R*, López CA, Tran TH*, Oppelstrup T, Bhatia H, Stanton LG, Zhang X, Sundram S, Di Natale F, Agarwal A, Dharuman G, Kokkila Schumacher SIL, Turbyville T*, Gulten G*, Van QN*, Goswami D*, Jean-Francois F*, Agamasu C*, Chen D*, Hettige JJ, Travers T, Sarkar S, Surh MP, Yang Y, Moody A, Liu S, Van Essen BC, Voter AF, Ramanathan A, Hengartner NW, Simanshu DK*, Stephen AG*, Bremer PT, Gnanakaran S, Glosli JN, Lightstone FC, McCormick F, Nissley DV*, Streitz FH
    Proc Natl Acad Sci U S A 2022
    PubMed PMID: 34983849
  • The tumor suppressor activity of DLC1 requires the interaction of its START domain with phosphatidylserine, PLCD1, and caveolin-1
    Sanchez-Solana B, Wang D, Qian X, Velayoudame P, Simanshu DK*, Acharya JK, Lowy DR
    Mol Cancer 2021
    PubMed PMID: 34727930
  • NMR 1H,13C, 15N backbone resonance assignments of the T35S and oncogenic T35S/Q61L mutants of human KRAS4b in the active, GppNHp-bound conformation
    Sharma AK*, Dyba M*, Tonelli M, Smith B*, Gillette WK*, Esposito D*, Nissley DV*, McCormick F, Maciag AE*
    Biomol NMR Assign 2021
    PubMed PMID: 34686998
  • Live tumor imaging shows macrophage induction and TMEM-mediated enrichment of cancer stem cells during metastatic dissemination
    Sharma VP, Tang B, Wang Y, Duran CL, Karagiannis GS, Xue EA, Entenberg D, Borriello L, Coste A, Eddy RJ, Kim G, Ye X, Jones JG, Grunblatt E, Agi N, Roy S, Bandyopadhyaya G, Adler E, Surve CR, Esposito D*, Goswami S, Segall JE, Guo W, Condeelis JS, Wakefield LM, Oktay MH
    Nat Commun 2021
    PubMed PMID: 34911937
  • Recapitulation of cell-like KRAS4b membrane dynamics on complex biomimetic membranes
    Shrestha R*, Chen D*, Frank P*, Nissley DV*, Turbyville TJ*
    iScience 2021
    PubMed PMID: 35106460
  • Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinant leucine-rich repeat proteins and protein complexes
    Snead K*, Wall V*, Ambrose H*, Esposito D*, Drew M*
    Protein Expr Purif 2022
    PubMed PMID: 35131438
  • mRNA vaccine-induced antibodies more effective than natural immunity in neutralizing SARS-CoV-2 and its high affinity variants
    Yu Y, Esposito D*, Kang Z, Lu J, Remaley AT, De Giorgi V, Chen LN, West K, Cao L
    Sci Rep 2022
    PubMed PMID: 35173254
  • RAS at 40: Update from the RAS initiative
    Nissley DV*, McCormick F
    Cancer Discov 2022
    PubMed PMID: 35046096
  • A structure is worth a thousand words: New insights for RAS and RAF regulation
    Simanshu DK*, Morrison DK
    Cancer Discov 2022
    PubMed PMID: 35046094
  • Precise characterization of KRAS4B proteoforms by combining immunoprecipitation with top-down mass spectrometry
    Adams LM, DeHart CJ*, Kelleher NL
    Methods Mol Biol 2021
    PubMed PMID: 33977470
  • RAS interaction with Sin1 is dispensable for mTORC2assembly and activity
    Castel P, Dharmaiah S*, Sale MJ, Messing S*, Rizzuto G, Cuevas-Navarro A, Cheng A, Trnka MJ, Urisman A, Esposito D*, Simanshu DK*, McCormick F
    Proc Natl Acad Sci U S A 2020
    PubMed PMID: 34380736
  • Theoretical classification of exchange geometries from the perspective of NMR relaxation dispersion
    Chao FA*, Zhang Y, Byrd RA
    J Magn Reson 2021
    PubMed PMID: 34058714
  • Refining the N-termini of the SARS-CoV-2 spike protein and its discrete receptor binding domain
    D’Ippolito RA*, Drew MR*, Mehalko J*, Snead K*, Wall V*, Putman Z*, Esposito D*, DeHart CJ*
    J Proteome Res 2021
    PubMed PMID: 34379411
  • Isoform- and phosphorylation-specific multiplexed quantitative pharmacodynamics of drugs targeting PI3K and MAPK signaling in xenograft models and clinical biopsies
    Herrick WG*, Kilpatrick CL*, Hollingshead MG, Esposito D*, O'Sullivan Coyne G, Gross AM, Johnson BC, Chen AP, Widemann BC, Doroshow JH, Parchment RE*, Srivastava AK*
    Mol Cancer Ther 2021
    PubMed PMID: 33536190
  • Serologic cross-reactivity of SARS-CoV-2 with endemic and seasonal betacoronaviruses
    Hicks J, Klumpp-Thomas C, Kalish H, Shunmugavel A, Mehalko J*, Denson JP*, Snead KR*, Drew M*, Corbett KS, Graham BS, Hall MD, Memoli MJ, Esposito D*, Sadtler K
    J Clin Immunol 2021
    PubMed PMID: 33725211
  • Undiagnosed SARS-CoV-2 seropositivity during the first 6 months of the COVID-19 pandemic in the United States
    Kalish H, Klumpp-Thomas C, Hunsberger S, Baus HA, Fay MP, Siripong N, Wang J*, Hicks J, Mehalko J*, Travers J, Drew M*, Pauly K, Spathies J, Ngo T, Adusei KM, Karkanitsa M, Croker JA, Li Y, Graubard BI, Czajkowski L, Belliveau O, Chairez C, Snead KR*, Frank P*, Shunmugavel A, Han A, Giurgea LT, Rosas LA, Bean R, Athota R, Cervantes-Medina A, Gouzoulis M, Heffelfinger B, Valenti S, Caldararo R*, Kolberg MM, Kelly A, Simon R, Shafiq S, Wall V*, Reed S, Ford EW, Lokwani R, Denson JP*, Messing S*, Michael SG, Gillette W*, Kimberly RP, Reis SE, Hall MD, Esposito D*, Memoli MJ, Sadtler K
    Sci Transl Med 2021
    PubMed PMID: 34158410
  • Energy penalties enhance flexible receptor docking in a model cavity
    Kamenik AS, Singh I, Lak P, Balius TE*, Liedl KR, Shoichet BK
    Proc Natl Acad Sci U S A 2021
    PubMed PMID: 34475217
  • Standardization of ELISA protocols for serosurveys of the SARS-CoV-2 pandemic using clinical and at-home blood sampling
    Klumpp-Thomas C, Kalish H, Drew M*, Hunsberger S, Snead K*, Fay MP, Mehalko J*, Shunmugavel A, Wall V*, Frank P*, Denson JP*, Hong M*, Gulten G, Messing S*, Hicks J, Michael S, Gillette W*, Hall MD, Memoli MJ, Esposito D*, Sadtler K
    Nat Commun 2021
    PubMed PMID: 33397956
  • Effect of D614G spike variant on immunoglobulin G, M, or A spike seroassay performance
    Klumpp-Thomas C, Kalish H, Hicks J, Mehalko J*, Drew M*, Memoli MJ, Hall MD, Esposito D*, Sadtler K
    J Infect Dis 2021
    PubMed PMID: 33257936
  • Sensitivity of oncogenic KRAS-expressing cells to CDK9 inhibition
    Lai LP*, Brel V, Sharma K*, Frappier J, Le-Henanf N, Vivet B, Muzet N, Schell E, Morales R, Rooney E, Basse N, Yi M*, Lacroix F, Holderfield M*, Englaro W, Marcireau C, Debussche L, Nissley DV*, McCormick F
    SLAS Discov 2021
    PubMed PMID: 33896272
  • A majority of uninfected adults show pre-existing antibody reactivity against SARS-CoV-2
    Majdoubi A, Michalski C, O'Connell SE, Dada S, Narpala SR, Gelinas JP, Mehta D, Cheung C, Winkler DF, Basappa M, Liu AC, Görges M, Barakauskas VE, Irvine MA, Mehalko J*, Esposito D*, Sekirov I, Jassem AN, Goldfarb DM, Pelech S, Douek DC, McDermott AB, Lavoie PM
    JCI Insight 2021
    PubMed PMID: 33720905
  • Production and membrane binding of N-terminally acetylated, C-terminally farnesylated and carboxymethylated KRAS4b
    Messing S*, Agamasu C*, Drew M*, DeHart CJ*, Stephen AG*, Gillette WK*
    Methods Mol Biol 2021
    PubMed PMID: 33977473
  • The metabolic landscape of RAS-driven cancers from biology to therapy
    Mukhopadhyay S*, Vander Heiden MG, McCormick F*
    Nat Cancer 2021
    PubMed PMID: 33870211
  • Coronavirus occurrence in the HIVE cohort of Michigan households: Reinfection frequency and serologic responses to seasonal and SARS coronaviruses
    Petrie JG, Bazzi LA, McDermott AB, Follmann D, Esposito D*, Hatcher C, Mateja A*, Narpala SR, O’Connell SE, Martin ET, Monto AS
    J Infect Dis 2021
    PubMed PMID: 33755731
  • Rapidly increasing SARS-CoV-2 seroprevalence and limited clinical disease in three Malian communities: a prospective cohort study
    Sagara I, Woodford J, Kone M, Assadou MH, Katile A, Attaher O, Zeguime A, Doucoure M, Higbee E, Lane J, Mohan R, Doritchamou J, Zaidi I, Esposito D*, Kwan J, Sadtler K, Dicko A, Duffy PE
    Clin Infect Dis 2021
    PubMed PMID: 34185847
  • NMR 1H,13C, 15N backbone resonance assignments of the T35S and oncogenic T35S/Q61L mutants of human KRAS4b in the active, GppNHp-bound conformation
    Sharma AK*, Dyba M*, Tonelli M, Smith B*, Gillette WK*, Esposito D*, Nissley DV*, McCormick F, Maciag AE*
    Biomol NMR Assign 2021
    PubMed PMID: 34686998
  • A simple colorimetric experiment using mammalian cell culture to study metabolism
    Sharma K*, Fizet KJ, Montgomery KR, Smeltzer NA , Sikorski MH,  Brown KG, Beyke BJ, Burkhart RC , Lynn AN, Grandinetti G
    Biomol NMR Assign 2021
    PubMed PMID: 32942341
  • Targeting eIF4A-dependent translation of KRAS signaling molecules
    Singh K, Lin J, Lecomte N, Mohan P, Gokce A, Sanghvi VR, Jiang M, Grbovic-Huezo O, Burčul A, Stark SG, Romesser PB, Chang Q, Melchor JP, Beyer RK*, Duggan M, Fukase Y, Yang G, Ouerfelli O, Viale A, de Stanchina E, Stamford AW, Meinke PT, Rätsch G, Leach SD, Ouyang Z, Wendel HG
    Cancer Res 2021
    PubMed PMID: 33632898
  • Towards quantitative and standardized serological and neutralization assays for COVID-19
    Tian L, Elsheikh EB, Patrone PN, Kearsley AJ, Gaigalas AK, Inwood S, Lin-Gibson S, Esposito D*, Wang L
    Int J Mol Sci 2021
    PubMed PMID: 33800363
  • KRAS interaction with RAF1 RAS-binding domain and cysteine-rich domain provides insights into RAS-mediated RAF activation
    Tran TH*, Chan AH*, Young LC, Bindu L*, Neale C, Messing S*, Dharmaiah S*, Taylor T*, Denson JP*, Esposito D*, Nissley DV*, Stephen AG*, McCormick F*, Simanshu DK*
    Nat Commun 2021
    PubMed PMID: 33608534
  • A practical guide to large-scale docking
    Bender BJ, Gahbauer S, Luttens A, Lyu J, Webb CM, Stein RM, Fink EA, Balius TE*, Carlsson J, Irwin JJ, Shoichet BK
    Nat Protoc 2021
    PubMed PMID: 34561691
  • A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells
    Grose C*, Putman Z*, Esposito D*
    Protein Expr Purif 2021
    PubMed PMID: 34087362
  • RAS nanoclusters: dynamic signaling platforms amenable to therapeutic intervention
    Van QN*, Prakash P, Shrestha R*, Balius TE*, Turbyville TJ*, Stephen AG*
    Biomolecules 2021
    PubMed PMID: 33802474
  • Uncovering a membrane-distal conformation of KRAS available to recruit RAF to the plasma membrane
    Van QN*, López CA, Tonelli M, Taylor T*, Niu B, Stanley CB, Bhowmik D, Tran TH*, Frank PH*, Messing S*, Alexander P*, Scott D, Ye X*, Drew M*, Chertov O*, Lösche M, Ramanathan A, Gross ML, Hengartner NW, Westler WM, Markley JL, Simanshu DK*, Nissley DV*, Gillette WK*, Esposito D*, McCormick F*, Gnanakaran S, Heinrich F, Stephen AG*
    Proc Natl Acad Sci U S A 2020
    PubMed PMID: 32913056
  • Structural Insights into the SPRED1-Neurofibromin-KRAS Complex and Disruption of SPRED1-Neurofibromin Interaction by Oncogenic EGFR
    Yan W*, Markegard E, Dharmaiah S*, Urisman A, Drew M*, Esposito D*, Scheffzek K, Nissley DV*, McCormick F*, Simanshu DK*
    Cell Rep 2020
    PubMed PMID: 32697994
  • Anionic Lipids Impact RAS-Binding Site Accessibility and Membrane Binding Affinity of CRAF RBD-CRD
    Travers T, López CA, Agamasu C*, Hettige JJ, Messing S*, García AE, Stephen AG*, Gnanakaran S
    Biophys J 2020
    PubMed PMID: 32649863
  • ssGSEA score-based Ras dependency indexes derived from gene expression data reveal potential Ras addiction mechanisms with possible clinical implications
    Yi M*, Nissley DV*, McCormick F*, Stephens RM*
    Sci Rep 2020
    PubMed PMID: 32581224
  • RAS internal tandem duplication disrupts GTPase-activating protein (GAP) binding to activate oncogenic signaling
    Nelson AC, Turbyville TJ*, Dharmaiah S*, Rigby M*, Yang R, Wang TY, Columbus J*, Stephens R*, Taylor T*, Sciacca D, Onsongo G, Sarver A, Subramanian S, Nissley DV*, Simanshu DK*, Lou E
    J Biol Chem 2020
    PubMed PMID: 32393580
  • The frequency of Ras mutations in cancer
    Prior IA, Hood FE, Hartley JL*
    Cancer Res 2020
    PubMed PMID: 32209560
  • Homogeneous dual-parametric coupled assay for simultaneous nucleotide exchange and KRAS/RAF-RBD interaction monitoring
    Kopra K, Vuorinen E, Abreu-Blanco M*, Wang Q, Eskonen V, Gillette W*, Pulliainen AT, Holderfield M*, Härmä H.
    Anal Chem 2020
    PubMed PMID: 32106676
  • The small molecule BI-2852 induces a nonfunctional dimer of KRAS.
    Tran TH*, Alexander P*, Dharmaiah S*, Agamasu C*, Nissley DV*, McCormick F*, Esposito D*, Simanshu DK*, Stephen AG*, Balius TE*
    Proc Natl Acad Sci U S A 2020
    PubMed PMID: 32047043
  • Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein
    Agamasu C*, Frank P*, Perkins S*, Waybright T*, Messing S*, Gillette W*, Stephen AG*
    J Vis Exp 2020
    PubMed PMID: 32009649
  • Membrane interactions of the globular domain and the hypervariable region of KRAS4b define its unique diffusion behavior
    Goswami D*, Chen D*, Yang Y, Gudla PR*, Columbus J*, Worthy K*, Rigby M*, Wheeler M*, Mukhopadhyay S*, Powell K*, Burgan W*, Wall V*, Esposito D*, Simanshu D*, Lightstone FC, Nissley DV*, McCormick F*, Turbyville T*
    Elife 2020
    PubMed PMID: 31958057
  • PDE6D Inhibitors with a New Design Principle Selectively Block K-Ras Activity
    Siddiqui FA, Alam C, Rosenqvist P, Ora M, Sabt A, Manoharan GB, Bindu L*, Okutachi S, Catillon M, Taylor T*, Abdelhafez OM, Lönnberg H, Stephen AG*, Papageorgiou AC, Virta P, Abankwa D
    ACS Omega 2019
    PubMed PMID: 31956834
  • Undermining glutaminolysis bolsters chemotherapy while NRF2 promotes chemoresistance in KRAS-driven pancreatic cancers
    Mukhopadhyay S*, Goswami D*, Adiseshaiah PP, Burgan W*, Yi M*, Guerin TM, Kozlov SV, Nissley DV*, McCormick F*
    Cancer Res 2020
    PubMed PMID: 31911550
  • Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer
    Sherekar M*, Han SW, Ghirlando R, Messing S*, Drew M*, Rabara D*, Waybright T*, Juneja P, O'Neill H, Stanley CB, Bhowmik D, Ramanathan A, Subramaniam S, Nissley DV*, Gillette W*, McCormick F*, Esposito D*
    J Biol Chem 2019
    PubMed PMID: 31836666
  • Atypical KRASG12R Mutant Is Impaired in PI3K Signaling and Macropinocytosis in Pancreatic Cancer.
    Hobbs GA, Baker NM, Miermont AM, Thurman RD, Pierobon M, Tran TH*, Anderson AO, Waters AM, Diehl JN, Papke B, Hodge RG, Klomp JE, Goodwin CM, DeLiberty JM, Wang J, Ng RWS, Gautam P, Bryant KL, Esposito D*, Campbell SL, Petricoin EF, Simanshu DK*, Aguirre AJ, Wolpin BM, Wennerberg K, Rudloff U, Cox AD, Der CJ.
    Cancer Discov 2019
    PubMed PMID: 31649109
  • AI Meets Exascale Computing: Advancing Cancer Research With Large-Scale High Performance Computing
    Bhattacharya T, Brettin T, Doroshow JH, Evrard YA, Greenspan EJ, Gryshuk AL, Hoang TT, Lauzon CBV, Nissley D*, Penberthy L, Stahlberg E, Stevens R, Streitz F, Tourassi G, Xia F, Zaki G
    Front Oncol 2019
    PubMed PMID: 31632915
  • KRAS G13D sensitivity to neurofibromin-mediated GTP hydrolysis
    Rabara D*, Tran TH*, Dharmaiah S*, Stephens RM*, McCormick F*, Simanshu DK*, Holderfield M*
    Proc Natl Acad Sci U S A 2019
    PubMed PMID: 31611389
  • Distinct Binding Preferences between Ras and Raf Family Members and the Impact on Oncogenic Ras Signaling
    Terrell EM, Durrant DE, Ritt DA, Sealover NE, Sheffels E, Spencer-Smith R, Esposito D*, Zhou Y, Hancock JF, Kortum RL, Morrison DK
    Mol Cell 2019
    PubMed PMID: 31606273
  • Second harmonic generation detection of Ras conformational changes and discovery of a small molecule binder
    Donohue E, Khorsand S, Mercado G, Varney KM, Wilder PT, Yu W, MacKerell AD Jr, Alexander P*, Van QN*, Moree B, Stephen AG*, Weber DJ, Salafsky J, McCormick F*
    Proc Natl Acad Sci U S A 2019 
    PubMed PMID: 31399543
  • PIP2 Influences the Conformational Dynamics of Membrane-Bound KRAS4b 
    McLean MA, Stephen AG*, Sligar SG
    Biochemistry 2019 
    PubMed PMID: 31339036
  • Organization of Farnesylated, Carboxymethylated KRAS4B on Membranes
    Barklis E, Stephen AG*, Staubus AO, Barklis RL, Alfadhli A
    J Mol Biol 2019
    PubMed PMID: 31330153
  • Structures of N-terminally processed KRAS provide insight into the role of N-acetylation 
    Dharmaiah S*, Tran TH*, Messing S*, Agamasu C*, Gillette WK*, Yan W*, Waybright T*, Alexander P*, Esposito D*, Nissley DV*, McCormick F*, Stephen AG*, Simanshu DK*
    Sci Rep 2019 
    PubMed PMID: 31324887
  • Blockade of leukemia inhibitory factor as a therapeutic approach to KRAS driven pancreatic cancer
    Wang MT, Fer N*, Galeas J, Collisson EA, Kim SE, Sharib J, McCormick F*
    Nat Commun 2019 
    PubMed PMID: 31296870
  • Membrane curvature sensing of the lipid-anchored K-Ras small GTPase
    Liang H, Mu H, Jean-Francois F*, Lakshman B*, Sarkar-Banerjee S, Zhuang Y, Zeng  Y, Gao W, Zaske AM, Nissley DV*, Gorfe AA, Zhao W, Zhou Y
    Life Sci Alliance 2019 
    PubMed PMID: 31296567
  • Intercellular Transfer of Oncogenic KRAS via Tunneling Nanotubes Introduces Intracellular Mutational Heterogeneity in Colon Cancer Cells
    Desir S, Wong P, Turbyville T*, Chen D*, Shetty M, Clark C, Zhai E, Romin Y, Manova-Todorova K, Starr TK, Nissley DV, Steer CJ, Subramanian S, Lou E
    Cancers (Basel) 2019
    PubMed PMID: 31247990
  • Production of Farnesylated and Methylated Proteins in an Engineered Insect Cell System
    Gillette W*, Frank P*, Perkins S*, Drew M*, Grose C*, Esposito D*
    Methods Mol Biol 2019
    PubMed PMID: 31152410
  • Feasibility of using NF1-GRD and AAV for gene replacement therapy in NF1-associated tumors
    Bai RY, Esposito D*, Tam AJ, McCormick F*, Riggins GJ, Wade Clapp D, Staedtke V
    Gene Ther 2019
    PubMed PMID: 31127187
  • New weapons to penetrate the armor: Novel reagents and assays developed at the NCI RAS Initiative to enable discovery of RAS therapeutics
    Esposito D*, Stephen AG*, Turbyville TJ*, Holderfield M*
    Semin Cancer Biol 2019
    PubMed PMID: 29432816
  • KRAS Prenylation Is Required for Bivalent Binding with Calmodulin in a Nucleotide-Independent Manner
    Agamasu C*, Ghirlando R, Taylor T*, Messing S*, Tran TH*, Bindu L*, Tonelli M, Nissley DV*, McCormick F*, Stephen AG*
    Biophys Journal 2019
    PubMed PMID: 30846362
  • Quantitative biophysical analysis defines key components modulating recruitment of the GTPase KRAS to the plasma membrane
    Lakshman B*, Messing S*, Schmid EM, Clogston JD, Gillette WK*, Esposito D*, Kessing B*, Fletcher DA, Nissley DV*, McCormick F*, Stephen AG*, Jean-Francois FL*
    J Biol Chem 2018
    PubMed ID: 30559287
  • GradientScanSurv-An exhaustive association test method for gene expression data with censored survival outcome
    Yi M*, Zhu R, Stephens RM*
    PloS One 2018
    PubMed ID: 30517129
  • Label-Free Time-Gated Luminescent Detection Method for the Nucleotides with Varying Phosphate Content
    Kopra K, Seppälä T, Rabara D*, Abreu-Blanco M*, Kulmala S, HolderfieldM*, Härmä H
    Sensors (Basel) 2018
    PubMed ID: 30453509
  • The bacterial Ras/Rap1 site-specific endopeptidase RRSP cleaves Ras through an atypical mechanism to disrupt Ras-ERK signaling
    Biancucci M, Minasov G, Banerjee A, Herrera A, Woida PJ, Kieffer MB, Bindu L*, Abreu-Blanco M*, Anderson WF, Gaponenko V, Stephen AG*, Holderfield M*, Satchell KJF
    Sci Signal  2018 
    PubMed ID: 30279169
  • Production of authentic geranylgeranylated KRAS4b using an engineered baculovirus system
    Procter L*, Grose C*, Esposito D*
    Protein Expr Purif 2018 
    PubMed ID: 29936133
  • Direct molecular dissection of tumor parenchyma from tumor stroma in tumor xenograft using mass spectrometry-based glycoproteomics
    Ye X*, Luke BT, Wei BR, Kaczmarczyk JA, Loncarek J, Dwyer JE, Johann DJ, Saul RG, Nissley DV*, McCormick F*, Whiteley GR*, Blonder J*
    Oncotarget 2018 
    PubMed ID: 29899869
  • Molecular recognition of RAS/RAF complex at the membrane: Role of RAF cysteine-rich domain
    Travers T, López CA, Van QN*, Neale C, Tonelli M, Stephen AG*, Gnanakaran S
    Sci Rep 2018
    PubMed ID: 29855542
  • Precise characterization of KRAS4b proteoforms in human colorectal cells and tumors reveals mutation/modification cross-talk
    Ntai I, Fornelli L, DeHart CJ, Hutton JE, Doubleday PF, LeDuc RD, van Nispen AJ, Fellers RT, Whiteley G*, Boja ES, Rodriguez H, Kelleher NL
    Proc Natl Acad Sci USA 2018
    PubMed ID: 29610327
  • Differential Effector Engagement by Oncogenic KRAS
    Yuan TL, Amzallag A, Bagni R*, Yi M*, Afghani S, Burgan W*, Fer N*, Strathern LA*, Powell K*, Smith B, Waters AM*, Drubin D, Thomson T, Liao R, Greninger P, Stein GT, Murchie E, Cortez E, Egan RK, Procter L*, Bess M, Cheng KT*, Lee CS, Lee LC, Fellmann C, Stephens R*, Luo J, Lowe SW, Benes CH, McCormick F*
    Cell Rep 2018
    PubMed ID: 29444439
  • New weapons to penetrate the armor: Novel reagents and assays developed at the NCI RAS Initiative to enable discovery of RAS therapeutics
    Esposito D*, Stephen AG*, Turbyville TJ*, Holderfield M*
    Semin Cancer Biol. 2018 
    PubMed ID: 29432816
  • Targeting RAS-driven human cancer cells with antibodies to upregulated and essential cell-surface proteins
    Martinko AJ, Truillet C, Julien O, Diaz JE, Horlbeck MA, Whiteley G*, Blonder J*, Weissman JS, Bandyopadhyay S, Evans MJ, Wells JA
    Elife 2018 
    PubMed ID: 29359686
  • K-Ras4B Remains Monomeric on Membranes over a Wide Range of Surface Densities and Lipid Compositions
    Chung JK, Lee YK, Denson JP*, Gillette WK*, Alvarez S, Stephen AG*, Groves JT
    Biophys J. 2018 
    PubMed ID: 29320680
  • Efforts to Develop KRAS Inhibitors
    Holderfield M*
    Cold Spring Harb Perspect Med 2018
    PubMed ID: 29101115
  • Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies
    Waters AM*, Ozkan-Dagliyan I, Vaseva AV, Fer N*, Strathern LA*, Hobbs GA, Tessier-Cloutier B, Gillette WK*, Bagni R*, Whiteley GR*, Hartley JL*, McCormick F*, Cox AD, Houghton PJ, Huntsman DG, Philips MR, Der CJ
    Sci Signal 2018
    PubMed ID: 28951536
  • RAS Proteins and Their Regulators in Human Disease
    Simanshu DK*, Nissley DV*, McCormick F*
    Cell 2017
    PubMed ID: 28666118
  • Tumor RAS Gene Expression Levels Are Influenced by the Mutational Status of RAS Genes and Both Upstream and Downstream RAS Pathway Genes
    Stephens RM*, Yi M*, Kessing B*, Nissley DV*, McCormick F*
    Cancer Inform 2017
    PubMed ID: 28634423
  • A KRAS GTPase K104Q Mutant Retains Downstream Signaling by Offsetting Defects in Regulation
    Yin G, Kistler S, George SD, Kuhlmann N, Garvey L*, Huynh M, Bagni RK*, Lammers M, Der CJ, Campbell SL
    J Biol Chem 2017
    PubMed ID: 28154176
  • Comparative proteomics of a model MCF10A-KRasG12V cell line reveals a distinct molecular signature of the KRasG12V cell surface
    Ye X, Chan KC, Waters AM*, Bess M, Harned A, Wei BR, Loncarek J, Luke BT, Orsburn BC, Hollinger BD*, Stephens RM*, Bagni R*, Martinko A, Wells JA, Nissley DV*, McCormick F*, Whiteley G*, Blonder J*
    Oncotarget 2016
    PubMed ID: 27894102
  • Inhibition of Ras/Raf/MEK/ERK Pathway Signaling by a Stress-Induced Phospho-Regulatory Circuit
    Ritt DA, Abreu-Blanco MT*, Bindu L*, Durrant DE, Zhou M, Specht SI, Stephen AG*, Holderfield M*, Morrison DK
    Mol Cell 2016
    PubMed ID: 27889448
  • Structural basis of recognition of farnesylated and methylated KRAS4b by PDEδ
    Dharmaiah S*, Bindu L*, Tran TH*, Gillette WK*, Frank PH*, Ghirlando R, Nissley DV*, Esposito D*, McCormick F*, Stephen AG*, Simanshu DK*
    Proc Natl Acad Sci USA 2016
    PubMed ID: 27791178
  • Single Synonymous Mutations in KRAS Cause Transformed Phenotypes in NIH3T3 Cells
    Waters AM*, Bagni R*, Portugal F*, Hartley JL*
    PLoS One 2016
    PubMed ID: 27684555
  • Farnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions
    Gillette WK*, Esposito D*, Abreu Blanco M*, Alexander P*, Bindu L*, Bittner C*, Chertov O*, Frank PH*, Grose C*, Jones JE*, Meng Z*, Perkins S*, Van Q, Ghirlando R, Fivash M*, Nissley DV*, McCormick F*, Holderfield M*, Stephen AG*
    Sci Rep 2015
    PubMed ID: 26522388
  • Progress in targeting RAF kinases for cancer therapy
    Turbyville TJ*, Holderfield M*
    Per Med 2015
    PubMed ID: 29771643
  • Dragging RAS back in the ring
    Stephen AG*, Esposito D*, Bagni RK*, McCormick F*
    Cancer Cell 2014
    PubMed ID: 24651010
  • Updated:

If you would like to reproduce some or all of this content, see Reuse of NCI Information for guidance about copyright and permissions. In the case of permitted digital reproduction, please credit the National Cancer Institute as the source and link to the original NCI product using the original product's title; e.g., “Publications from the RAS Initiative was originally published by the National Cancer Institute.”