DCB Research Resources
DCB supports a variety of resources that are available to scientists studying cancer to aid them in their research. Descriptions of these resources and information on how to access them are included below. Along with research tools, DCB shares presentations from the annual DCB New Grantee Workshop about NCI grants processes for new and early stage investigators as well as information about the NIH Citizen Science Working Group.
The NCI Mouse Repository
The NCI Mouse Repository is an NCI-funded resource for mouse cancer models and associated strains. The repository makes strains available to all members of the scientific community (academic, non-profit, and commercial). NCI Mouse Repository strains are cryoarchived and distributed as frozen germoplasm (embryos and/or sperm).
MicroRNAs (miRNAs) are small non-coding RNA molecules known to play an important role in fundamental cellular processes through negative post-transcriptional regulation of gene expression. In an effort to address the role that microRNAs play in human cancer, their use as diagnostic tools, and their potential function as new targets for therapeutic intervention in the treatment of cancer, the Division of Cancer Biology (DCB) of the National Cancer Institute (NCI) has supported the generation of mouse embryonic stem cells (mESCs) harboring most known mouse miRNAs. In order to enhance novel information obtained on their role in cancer, 1501 genetically engineered mESC lines were produced, harboring conditional microRNA transgenes.
For more information, please visit the NCI Mouse Repository website.
GM/CA X-ray Beamline
NCI partnered with the National Institute of General Medical Sciences (NIGMS) to fund the construction and management of a state-of-the-art macromolecular crystallography synchrotron beamline for determining structures of biologically important macromolecules. The beamline, GM/CA CAT, is located at the Advanced Photon Source on the grounds of Argonne National Lab just outside of Chicago.
NCI has a significant amount of beamtime dedicated for the use of its grantees. Investigators interested in taking experiments to this facility should contact the beamline directly or email Dr. J. Randy Knowlton for additional information.
Chernobyl Tissue Bank (CTB)
DCB supports and manages biospecimen resources that collect, store, process, and disseminate human biological specimens (biospecimens) and associated data set for research on human cancer biology. The Chernobyl Tissue Bank is an international collaborative project that is supported by NCI and another global partner, with active participation from Russia and Ukraine, two countries heavily affected by the 1986 Chernobyl accident. The objective of the CTB is to establish and maintain a research resource that supports studies on the biology of thyroid cancer, the major health consequence of the Chernobyl accident.
For more information on this Tissue Bank, please visit the Chernobyl Tissue Bank website.
The International Registry of Werner Syndrome
The International Registry of Werner Syndrome is the primary repository of samples and data from patients with Werner Syndrome (WS), and was established in 1988 as part of an objective to positionally clone the WS gene.
The registry ascertains and genotypes new pedigree cases from around the world, using lymphoblastoids and/or fibroblasts from human research participants, and provides genetic confirmation of classical WS. It also establishes and cryopreserves cell lines and other material from these pedigrees (both affected patients and their clinically unaffected siblings), including Epstein-Barr transformed peripheral blood B lymphocytes, primary skin fibroblasts, immortalized skin fibroblasts, WRN cDNA constructs, and others. All these materials are available for research.
For more information on this Registry, please visit the Werner Syndrome Registry website.
NIH MHC Tetramer Program
This core facility provides custom synthesis and distribution of soluble MHC-peptide tetramer reagents that can be used to stain antigen-specific T cells. The facility is supported by a contract from the National Institute of Allergy and Infectious Diseases, with steering committee participation from NCI through DCB.
For more information about this program, please visit the Emory University tetramer website.
Reagents Available to NCI-funded Researchers
The Tumor Microenvironment Network (TMEN) generated a number of resources that are now available to NCI-funded cancer researchers. These resources include:
- EHS (Engelbreth-Holm-Swarm) sarcoma-derived laminin rich matrix
- A pool of EHS sarcoma cell-derived extracellular matrix available for those who work on three-dimensional models to study tumor-host interactions.
- Novel antibodies to detect cancer stem cells and stromal cells
- CD10-PE/Cy5, CD140b-Biotin, CD16-PE/Cy5, CD18-PE/Cy5, CD20-PE/Cy5, CD24-FITC, CD24-PE, CD2-PE/Cy5, CD31-Biotin, CD3-PE/Cy5, CD44-APC, CD44-PE, CD45-PE/Cy5, CD45-PE/Cy7, CD64-Biotin, EGFR-FITC, EGFR-PE, EpCAM-APC, EpCAM-FITC, EpCAM-PE, H2Kd-Biotin, mCD45-PE/Cy5, SAV-PE/Cy5, SAV-PE/Cy7
- Human xenograft tumor bank with characterized stem cell populations
- A tumor bank of characterized human breast and colon solid tumors containing xenografts. Vials of frozen tumor cells from each tumor type have been stored for use. Recipients would be responsible for amplifying the stem cells and serving as a central bank for their institution as well as other investigators outside their institution.
- Bone Marrow Derived Cells from donor mice
- This bank contains bone marrow cells from C57BL/6J and C57BL/6-Tg-(UBC-GFP) 30 Scha/J mouse lines in cryopreserved aliquots that can be re-infused as needed.
- RCAS Constructs
- RCAS(A)-GFP – An avian retroviral vector for GFP expression in TVa mice. The vector needs to be introduced in DF1 cells for virus generation. Normally the DF1 cells themselves are introduced into mice directly.
- RCAS(B)-DsRed - An avian retroviral vector for DsRed expression in TVb mice. The vector needs to be introduced in DF1 cells for virus generation. Normally the DF1 cells themselves are introduced into mice directly.
Researchers interested in obtaining these reagents should contact Jeff Hildesheim, Division of Cancer Biology, NCI, for additional information and a reagent request form.
The Physical Sciences - Oncology Network Bioresource Core Facility (PBCF)
The Physical Sciences - Oncology Network Bioresource Core Facility (PBCF) is an NCI-funded resource at ATCC housing an authenticated set of non-malignant and cancerous cell lines, cell culture reagents, and related standard operating protocols available to all NIH-funded cancer researchers.
Features of the PBCF include:
• Free cost of each cell line and reagents (shipping & handling fees apply)
• Each batch of cell lines originates from the same lot number and passage number, making their use ideal for collaborative studies
• 41 distinct cell lines are available from 8 cancer types and non-malignant counterparts, all with detailed culture SOPs
• First shipment of each cell line includes a media/reagent starter kit (if requested)
• Genomic, proteomic, and physical characterization data for many of the cell lines are available.
Datasets of published work using the PBCF cell lines should be reported to the PBCF and the NCI. For information on the list of cell lines available and order forms, please visit the NCI Physical Sciences-Oncology Network Bioresource webpage or email Nastaran Zahir with questions.
New Grantee Workshop
DCB offers an annual workshop for new and early-stage investigators to familiarize them with the processes and standards of DCB, NCI and NIH.
The workshop is designed for DCB grantees who have received their first independent NIH grant within the last year. New grantees are identified by the Division, and attendance is by invitation only.
The workshop covers:
- Structure, roles, and inter-relationships of NIH, NCI, and DCB
- Branches within DCB
- NIH peer review process
- Competitive renewal applications
- Sources of supplemental funding
- Grantee responsibilities in managing their awards
- Research Resources
The workshop includes breakout sessions with DCB Program Directors and a seminar from an NCI-funded mid-career cancer researcher.
Questions should be directed to Dr. Susan McCarthy.
PDFs of the presentations from the January 2019 Workshop (Posted with permission from each author; please do not use without consent):
- New Grantee Workshop 2019 Agenda
- Introduction to the NIH Grants System and Division of Cancer Biology
- NCI Budget Process
- Communicating with Your NCI Program Director: It's a Two Way Street
- Post-Award Administration
- Engagement and Funding Opportunities
- NCI Resources for Researchers
- NCI Research Training and Career Development Awards
- CRCHD: Diversity Training and Cancer Health Disparities Research Opportunities
- Now you have your first R01, what’s next?
- NIH Review: Insights for Established Investigators
Biomedical Citizen Science and Crowdsourcing: The NIH Citizen Science Working Group
This trans-NIH working group is investigating the usefulness and possible incorporation of citizen science methodologies into biomedical research in a way that maintains NIH’s high scientific and ethical standards.
Citizen science is a collaborative approach to research involving the public as direct collaborators and partners in the research process itself — not just as subjects of the research or advisors to the research. Citizen science takes many forms, and involves a variety of approaches benefiting from the creativity and problem-solving skills of the public and from citizen-collected data and insights not obtainable through conventional approaches.
This working group investigates, shares best practices, and engages in discussion with other agencies and groups promoting citizen science in other fields. The working group is composed of program officers, scientific review officers, and others from across NIH interested in furthering the adoption and incorporation of citizen science methodology into biomedical research.